Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0068(Opioid peptide)

DFBP ID	DFBPOPIO0068
Peptide sequence	TTMPLW
Type	Native peptide
Peptide/Function name	Opioid peptide

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], Antihypertensive activity ^[D2], DPP IV-inhibitory activity ^[D3], Immunomodulatory activity ^[D4], Multifunctional activity ^[D5]

Calculated physicochemical properties

Three-letter amino acid

Thr-Thr-Met-Pro-Leu-Trp

Single-letter amino acid

TTMPLW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	747.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.3000^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine
Precursor protein	α_s1-Casein
Residue position	f(194-199)
Precursor protein(s) search	Source.1: DFBPPR7688 ---- Milk proteins ---- Alpha-S1-casein Source.2: DFBPPR7717 ---- Milk proteins ---- Alpha-S1-casein Source.3: DFBPPR8488 ---- Milk proteins ---- Alpha-S1-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity	The peptide showed opioid activity (data not shown).
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Delipidated caseins were digested by non-pretreated trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPACEI0575
[D2]	DFBPANHY0140, DFBPANHY0974
[D3]	DFBPDPIV0188
[D4]	DFBPIMMU0029
[D5]	DFBPMUFU0137

BIOPEP-UWM

[D6]

3127, 3530, 8172, 9619

APD

[D7]

BioPepDB

[D8]

MBPDB

[D9]

Reference(s)

Primary literature	Migliore-Samour D, Floc'h F, Jollès P. Biologically active casein peptides implicated in immunomodulation. J Dairy Res. 1989;56(3):357-62. PMID: 2668358 DOI: 10.1017/s0022029900028806
Other literature(s)	[1] Fiat A M, Jollès P. Caseins of various origins and biologically active casein peptides and oligosaccharides: Structural and physiological aspects[J]. Molecular & Cellular Biochemistry, 1989, 87(1):5-30.
PubDate	1989