Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0073(Opioid peptide)

DFBP ID	DFBPOPIO0073
Peptide sequence	YPSF
Type	Native peptide
Peptide/Function name	Opioid peptide, β-Casomorphin

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Ser-Phe

Single-letter amino acid

YPSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	512.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.2250^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protien
Precursor protein	β-Casein
Residue position	f(41-44)
Precursor protein(s) search	Source.1: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.2: DFBPPR1535 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.3: DFBPPR1863 ---- Plant proteins ---- Chitinase 6 Source.4: DFBPPR2061 ---- Plant proteins ---- Probable ion channel POLLUX Source.5: DFBPPR3076 ---- Plant proteins ---- GTP-binding nuclear protein Ran-1 Source.6: DFBPPR3539 ---- Plant proteins ---- GTP-binding nuclear protein Ran-2 Source.7: DFBPPR3554 ---- Plant proteins ---- GTP-binding nuclear protein Ran-3 Source.8: DFBPPR3682 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 5 Source.9: DFBPPR4181 ---- Plant proteins ---- Acylamino-acid-releasing enzyme 1 Source.10: DFBPPR4852 ---- Plant proteins ---- B3 domain-containing protein Os01g0905400 Source.11: DFBPPR5047 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.12: DFBPPR5563 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.13: DFBPPR5791 ---- Plant proteins ---- Uroporphyrinogen decarboxylase, chloroplastic Source.14: DFBPPR6274 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.15: DFBPPR6292 ---- Plant proteins ---- Probable ion channel SYM8 Source.16: DFBPPR6696 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.17: DFBPPR7065 ---- Plant proteins ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.18: DFBPPR7441 ---- Plant proteins ---- Defensin-like protein 4 Source.19: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.20: DFBPPR7744 ---- Plant proteins ---- Maturase K Source.21: DFBPPR16208 ---- Animal proteins ---- Ammonium transporter Rh type B Source.22: DFBPPR16222 ---- Animal proteins ---- Sodium- and chloride-dependent taurine transporter Source.23: DFBPPR16269 ---- Animal proteins ---- Ammonium transporter Rh type C Source.24: DFBPPR16440 ---- Animal proteins ---- Inactive rhomboid protein 2 Source.25: DFBPPR17136 ---- Animal proteins ---- Tyrosine-protein kinase Fyn Source.26: DFBPPR19020 ---- Animal proteins ---- Ammonium transporter Rh type B Source.27: DFBPPR19173 ---- Animal proteins ---- Carbonic anhydrase 1 Source.28: DFBPPR20674 ---- Animal proteins ---- Stromal membrane-associated protein 2 Source.29: DFBPPR8752 ---- Animal proteins ---- Tyrosine-protein kinase Fyn Source.30: DFBPPR9378 ---- Animal proteins ---- Ammonium transporter Rh type C Source.31: DFBPPR9446 ---- Animal proteins ---- Ammonium transporter Rh type B Source.32: DFBPPR10255 ---- Animal proteins ---- Tyrosine-protein kinase Fyn Source.33: DFBPPR11530 ---- Animal proteins ---- Probable cation-transporting ATPase 13A4 Source.34: DFBPPR11622 ---- Animal proteins ---- Ammonium transporter Rh type C Source.35: DFBPPR11921 ---- Animal proteins ---- GATOR complex protein WDR59 Source.36: DFBPPR12839 ---- Animal proteins ---- Ammonium transporter Rh type B Source.37: DFBPPR12942 ---- Animal proteins ---- Ammonium transporter Rh type C Source.38: DFBPPR13154 ---- Animal proteins ---- Annexin A1 Source.39: DFBPPR13697 ---- Animal proteins ---- Carbonic anhydrase 1 Source.40: DFBPPR14331 ---- Marine protein ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.41: DFBPPR14539 ---- Marine protein ---- Aryl hydrocarbon receptor nuclear translocator Source.42: DFBPPR14683 ---- Marine protein ---- Ammonium transporter Rh type C Source.43: DFBPPR14994 ---- Microorganism protein ---- Serine/threonine-protein kinase MEC1 Source.44: DFBPPR15190 ---- Microorganism protein ---- Pescadillo homolog Source.45: DFBPPR15259 ---- Microorganism protein ---- Guanidinobutyrase Source.46: DFBPPR7772 ---- Plant protein ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.47: DFBPPR7966 ---- Plant protein ---- GTP-binding nuclear protein Ran/TC4 Source.48: DFBPPR7992 ---- Plant protein ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.49: DFBPPR8035 ---- Plant protein ---- Endochitinase Source.50: DFBPPR8044 ---- Plant protein ---- Photosystem I P700 chlorophyll a apoprotein A1 Source.51: DFBPPR8058 ---- Plant protein ---- Endochitinase CH5B Source.52: DFBPPR8224 ---- Plant protein ---- Photosystem I P700 chlorophyll a apoprotein A1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity

The active form of the peptide YPSF was YPSF-NH₂. It was specific ligands for the u-receptor and showed weak opioid activity. As shown in table 1.

Table 1 Opioid activity of peptides
Peptide	IC₅₀ (uM)			IC₅₀ MVD	Activity	References
	RA^a	GPI^b	MVD^c	IC₅₀ GPI
YPSF-NH₂	35	30	> 1000	－	Opioid agonist	—
	-receptor					[1]

^a Radioreceptor assay in the presence of 1 nM [³H]naloxone.
^b Guinea pig ileum assay.
^c Mouse vas deferens assay. nd, not determined

Specific target protein(s)

Specific Target Protein(s):
Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Synthesis peptide
Enzyme(s)/starter culture	Peptide was synthesized by a solid-phase method on chloromethyl resin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yoshikawa, M., Tani, F., Yoshimura, T., Chiba, H. Opioid Peptides from Milk Proteins. Agricultural and Biological Chemistry. 2014, 50, 2419-21. DOI: 10.1080/00021369.1986.10867763
Other literature(s)	[1] Chiba H, Yoshikawa M. Biologically functional peptides from food proteins: new opioid peptides from milk proteins[J]. Research, 1986.
PubDate	2014