Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0079(Opioid peptide)

DFBP ID	DFBPOPIO0079
Peptide sequence	TSKYR
Type	Native peptide
Peptide/Function name	Opioid peptide, Neokyotorphin

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	Antimicrobial activity ^[D1], Anticancer activity ^[D2], Regulating activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Thr-Ser-Lys-Tyr-Arg

Single-letter amino acid

TSKYR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	653.73 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.41 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.2400^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine
Precursor protein	Bovine brain
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16246 ---- Animal proteins ---- Hemoglobin subunit alpha Source.2: DFBPPR17468 ---- Animal proteins ---- Hemoglobin subunit alpha Source.3: DFBPPR9179 ---- Animal proteins ---- Hemoglobin subunit alpha Source.4: DFBPPR10736 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A1 Source.5: DFBPPR11603 ---- Animal proteins ---- Syntaxin-binding protein 1 Source.6: DFBPPR12431 ---- Animal proteins ---- Hemoglobin subunit alpha-1/2 Source.7: DFBPPR12743 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A-like 1 Source.8: DFBPPR13199 ---- Animal proteins ---- Hemoglobin subunit alpha Source.9: DFBPPR13468 ---- Animal proteins ---- Hemoglobin subunit alpha-1 Source.10: DFBPPR13476 ---- Animal proteins ---- Hemoglobin subunit alpha-2 Source.11: DFBPPR13661 ---- Animal proteins ---- Hemoglobin subunit alpha-1/2 Source.12: DFBPPR14097 ---- Marine protein ---- Katanin p60 ATPase-containing subunit A1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity	The peptide showed opioid activity.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPAMIC0286
[D2]	DFBPANCA0863
[D3]	DFBPREPE0007
[D4]	DFBPMUFU0707

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Takagi H, Shiomi H, Fukui K, Hayashi K, Kiso Y, Kitagawa K. Isolation of a novel analgesic pentapeptide, neo-kyotorphin, from bovine brain. Life Sci. 1982 Oct 18-25;31(16-17):1733-6. PMID: 7154833 DOI: 10.1016/0024-3205(82)90197-7
Other literature(s)	[1] Catiau L, Traisnel J, Delvaldubois V, et al. Minimal antimicrobial peptidic sequence from hemoglobin alpha-chain: KYR.[J]. Peptides, 2011, 32(4):633-638.
PubDate	1982