Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0101(Opioid peptide)

DFBP ID	DFBPOPIO0101
Peptide sequence	YPVEPF
Type	Native peptide
Peptide/Function name	Opioid peptide, Neocasomorphin-6

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	DPP IV-inhibitory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Val-Glu-Pro-Phe

Single-letter amino acid

YPVEPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	750.84 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.1667^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	β-Casein
Residue position	f(114-119)
Precursor protein(s) search	Source.1: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Opioid activity

Opioid activity	References
The activity of this peptide in the guinea pig ileum (GPI, μ-opiate receptor) assay was IC₅₀ = 56 μM and the binding affinity for rat brain μ-opioid receptor was IC₅₀ = 92 μM.	—
Opioid agonist, (μ-opiate receptor)	[1]

Specific target protein(s)

Specific Target Protein(s):
Mu-type opioid receptor

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	To simulate in vivo digestion, bovine β-casein was digested by pepsin, pancreatin (or chymotrypsin) and then by leucine aminopeptidase (LAP).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPDPIV0109
[D2]	DFBPMUFU0728

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Jinsmaa Y, Yoshikawa M. Enzymatic release of neocasomorphin and beta-casomorphin from bovine beta-casein. Peptides. 1999;20(8):957-62. PMID: 10503774 DOI: 10.1016/s0196-9781(99)00088-1
Other literature(s)	[1] Teschemacher H. Opioid receptor ligands derived from food proteins.[J]. Current Pharmaceutical Design, 2003, 9(16):-.
PubDate	1999