Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPOPIO0112(Opioid peptide)

DFBP ID	DFBPOPIO0112
Peptide sequence	VYPFPGPI
Type	Native peptide
Peptide/Function name	Opioid peptide, [Val⁵⁹]-Casomorphin-7^[3]

Function-activity relationship

Main bioactivity	Opioid activity
Otheir bioactivity	ACE-inhibitory activity ^[D1], PEP-inhibitory activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile

Single-letter amino acid

VYPFPGPI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	889.05 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6250^c

Hydrophilic residue ratio

87.5%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine milk protein
Precursor protein	β-Casein
Residue position	f(59-66)
Precursor protein(s) search	Source.1: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	Link 1: DFBPOPIO0113----Ovine milk protein----β-Casein Link 2: DFBPOPIO0114----Buffalo milk protein----β-Casein

Biological/Functional activity & target protein

Opioid activity

Opioid activity	References
Opioid agonist	[1]
The results have shown that the opioid activity of [Val⁰]-β-CMs was weaker than that of β-CMs and the former could be converted to the latter by leucine aminopeptidase (LAP).	[3]

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	To simulate in vivo digestion, bovine β-casein was digested by pepsin and then by pancreatin^[3].

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) The peptide was also found in Ovine, Buffalo (β-casein).
(2) The sequence of bovine β-casein (59-81) is -LVYPFPGPI-Pro⁶⁷/His⁶⁷-NSLPQNIPPLTQTP- ^[3].

Database cross-references

DFBP

[D1]	DFBPACEI0666, DFBPACEI1138, DFBPACEI1139
[D2]	DFBPPEPI0011, DFBPPEPI0046, DFBPPEPI0047
[D3]	DFBPMUFU0150

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Brantl V, Teschemacher H, Henschen A, Lottspeich F. Novel opioid peptides derived from casein (beta-casomorphins). I. Isolation from bovine casein peptone. Hoppe Seylers Z Physiol Chem. 1979 Sep;360(9):1211-6. PMID: 511110 DOI: 10.1515/bchm2.1979.360.2.1211
Other literature(s)	[1] Kostyra E, Sienkiewiczsz┅apka E, Jarmo┅owska B, et al. Opioid peptides derived from milk proteins.[J]. Polish Journal of Food & Nutrition Sciences, 2004. [2] Asano M, Nio N, Ariyoshi Y. Inhibition of prolyl endopeptidase by synthetic beta-casein peptides and their derivatives with a C-terminal prolinol or prolinal[J]. Journal of the Agricultural Chemical Society of Japan, 1992, 56(6):976-977. [3] Jinsmaa Y, Yoshikawa M. Enzymatic release of neocasomorphin and beta-casomorphin from bovine beta-casein.[J]. Peptides, 1999, 20(8):957.
PubDate	1979