Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPPEPI0071(PEP-inhibitory peptide)

DFBP ID	DFBPPEPI0071
Peptide sequence	VEPIPY
Type	Native peptide
Peptide/Function name	PEP-inhibitory peptide

Function-activity relationship

Main bioactivity	PEP-inhibitory activity
Otheir bioactivity	Immunomodulatory activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Val-Glu-Pro-Ile-Pro-Tyr

Single-letter amino acid

VEPIPY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	716.82 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.1167^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(54-59)
Precursor protein(s) search	Source.1: DFBPPR7605 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

PEP-inhibitory activity	Inhibitor of Prolyl endopeptidase (EC:3.4.21.26). The peptide possibly act as anti-amnestic agents. The peptide was assayed in vitro for their capacity to inhibit the Flavobacterium meningosepticum PEP activity, the IC₅₀ value is 180 uM and it's moderately active.
Specific target protein(s)	Specific Target Protein(s): Prolyl endopeptidase

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Synthetic peptide
Enzyme(s)/starter culture	No enzyme

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPIMMU0001, DFBPIMMU0053
[D2]	DFBPMUFU0755

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Asano M, Nio N, Ariyoshi Y. Inhibition of prolyl endopeptidase by synthetic peptide fragments of human beta-casein. Agric Biol Chem. 1991 Mar;55(3):825-8. PMID: 1368631
Other literature(s)	N.D
PubDate	1991