Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPREPE0007(Regulating peptide)

DFBP ID	DFBPREPE0007
Peptide sequence	TSKYR
Type	Native peptide
Peptide/Function name	Regulating peptide, Neokyotorphin

Function-activity relationship

Main bioactivity	Regulating activity
Otheir bioactivity	Antimicrobial activity ^[D1], Anticancer activity ^[D2], Opioid activity ^[D3], Multifunctional activity ^[D4]

Calculated physicochemical properties

Three-letter amino acid

Thr-Ser-Lys-Tyr-Arg

Single-letter amino acid

TSKYR

Peptide length

Peptide mass

Experimental mass	Theoretical mass
638.703 Da	653.73 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.41 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.2400^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Hibernating ground squirrel
Precursor protein	The brain of hibernating ground squirrel
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16246 ---- Animal proteins ---- Hemoglobin subunit alpha Source.2: DFBPPR17468 ---- Animal proteins ---- Hemoglobin subunit alpha Source.3: DFBPPR9179 ---- Animal proteins ---- Hemoglobin subunit alpha Source.4: DFBPPR10736 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A1 Source.5: DFBPPR11603 ---- Animal proteins ---- Syntaxin-binding protein 1 Source.6: DFBPPR12431 ---- Animal proteins ---- Hemoglobin subunit alpha-1/2 Source.7: DFBPPR12743 ---- Animal proteins ---- Katanin p60 ATPase-containing subunit A-like 1 Source.8: DFBPPR13199 ---- Animal proteins ---- Hemoglobin subunit alpha Source.9: DFBPPR13468 ---- Animal proteins ---- Hemoglobin subunit alpha-1 Source.10: DFBPPR13476 ---- Animal proteins ---- Hemoglobin subunit alpha-2 Source.11: DFBPPR13661 ---- Animal proteins ---- Hemoglobin subunit alpha-1/2 Source.12: DFBPPR14097 ---- Marine protein ---- Katanin p60 ATPase-containing subunit A1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Regulating activity

Ion flow regulating peptide neokyotorphin (TSKYR), earlier known as a weak analgetic, is found to have a cardiotropic and thermoregulatory activity. Neokyotorphin activates in vitro voltage-dependent calcium and blocks ATP-dependent potassium currents in the frog atrial fibres. Intraperitoneal injection of this peptide in hibernating ground squirrels speeds up the arousal of animals increasing sharply the heart rate and oxygen consumption. Intraperitoneal and intranasal administrations of neokyotorphin in rats raises body temperature in thermoneutral conditions (26-28 degrees C) exerting no effects at low (4-6 degrees C) and high (31-32 degrees C) environmental temperatures.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	Synthesis peptide

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPAMIC0286
[D2]	DFBPANCA0863
[D3]	DFBPOPIO0079
[D4]	DFBPMUFU0707

[D5]

[D6]

[D7]

[D8]

Reference(s)

Primary literature	Ziganshin RKh, Sviriaev VI, Vas'kovskiĭ BV, Mikhaleva II, Ivanov VT, Kokoz IuM, Alekseev AE, Korystova AF, Sukhova GS, Emel'ianova TG, et al. Biologicheski aktivnye peptidy, bydelennye iz mozga zimospiashchikh suslikov [Biologically active peptides isolated from the brain of hibernating ground squirrels]. Bioorg Khim. 1994 Aug-Sep;20(8-9):899-918. PMID: 7826417
Other literature(s)	N.D
PubDate	1994