The release of vasoactive substances, 6-keto-prostaglandin F1α (stable marker of prostaglandin I2), was significantly influenced by the incubation with the peptide EEE compared with control cells (P < 0.05), as shown in Table 1.
Table 1 Relative concentrations of vasoactive substances per milligram of cell protein in cell medium of human aortic endothelial cells treated with various di- and tripeptides compared to untreated cells a.
Peptides
| Concentration | Relative concentration of vasoactive substances (% of control)
| TXB2 (control = 100 ± 11)
| 6-keto-PGF1α (control = 105 ± 15)
| ET-1 (control = 100 ± 19)
| NO (control = 100 ± 29)
| H-Glu-Glu-Glu-OH
| 100 nM | 121 ± 37
| 183 ± 41*
| 101 ± 17
| 94 ± 8 | 50 μM | 105 ± 43
| 122 ± 32
| 97 ± 14
| 110 ± 37
| a Results are means ± SD for n=6. * Different from untreated cells; P < 0.05.
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