The release of vasoactive substances, Thromboxan B2 (stable marker of thromboxan A2), was significantly influenced by the incubation with the peptide LLL compared with control cells (P < 0.05), as shown in Table 1.
Table 1 Relative concentrations of vasoactive substances per milligram of cell protein in cell medium of human aortic endothelial cells treated with various di- and tripeptides compared to untreated cells a.
Peptides
| Concentration | Relative concentration of vasoactive substances (% of control)
| TXB2 (control = 100 ± 11)
| 6-keto-PGF1α (control = 105 ± 15)
| ET-1 (control = 100 ± 19)
| NO (control = 100 ± 29)
| H-Leu-Leu-Leu-OH
| 100 nM | 97 ± 33
| 106 ± 56
| 94 ± 3
| 108 ± 8 | 50 μM | 160 ± 42*
| 158 ± 60
| 94 ± 7
| 96 ± 10
| a Results are means ± SD for n=6. * Different from untreated cells; P < 0.05.
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