DFBP ID - DFBPSTPE0010(Stimulating peptide)

DFBP ID DFBPSTPE0010 Peptide sequence CPQLQPQNPSQQQPQEQG Type Native peptide Peptide/Function name Stimulating peptide

Function-activity relationship

Main bioactivity Stimulating activity Otheir bioactivity N.D

Calculated physicochemical properties

Three-letter amino acid Cys-Pro-Gln-Leu-Gln-Pro-Gln-Asn-Pro-Ser-Gln-Gln-Gln-Pro-Gln-Glu-Gln-Gly Single-letter amino acid CPQLQPQNPSQQQPQEQG Peptide length 18 Peptide mass Experimental mass Theoretical mass N.D 2035.20 Da c Net charge 0.00 c Isoelectric point (pI) 3.34 c IC50 N.D pIC50 N.D GRAVY -2.0167 c Hydrophilic residue ratio 33.33% c Peptide calculator To calculate the physicochemical properties of bioactive peptide.

Peptide source & Food-borne protein(s) search

Classification Plant Organism/Source Wheat Precursor protein Alpha-gliadin Residue position f(6-21) Precursor protein(s) search No matching precursor protein found Link-research Inductive analysis There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Stimulating activity Stimulating gamma-interferon production, as shown in Table 1. Table 1 Gamma-interferon production (IU/ml) in blood from patients with coeliac disease and controls in response to fractions derived from gliadin and to synthetic peptides. Peptide Coeliac patients Controls 1 2 3 4 5 1 2 3 4 5 CPQLQPQNPSQQQPQEQG 0.2 0.2 0.2, 0.1 Blood (1 ml) was incubated for 24 h at 37°C with fraction or peptide (0.5 mg in 0.1 ml PBS). Note: Blanks (no peptide) were all less than 0.2 IU/ml. Specific target protein(s) N.D

Taste properties & Structure

Bitterness Literature report N.D Bitter prediction tools Non-bitter taste prediction SMILES N[C@@]([H])(CS)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)NCC(=O)O

Preparation method

Mode of preparation Synthesis Enzyme(s)/starter culture Synthesis peptide

Stability & Cytotoxicity

Peptide stability Literature report: N.D EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool) Peptide cytotoxicity Literature report: N.D Prediction: ToxinPred

Additional information

Additional information N.D

Database cross-references

BIOPEP-UWM [D1] 3646

APD [D2] -

BioPepDB [D3] -

MBPDB [D4] -

Reference(s)

Primary literature Cornell HJ, Skerritt JH, Puy R, Javadpour M. Studies of in vitro gamma-interferon production in coeliac disease as a response to gliadin peptides. Biochim Biophys Acta. 1994 May 25;1226(2):126-30. PMID: 8204658 DOI: 10.1016/0925-4439(94)90019-1 Other literature(s) N.D PubDate 1994