Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPSTPE0021(Stimulating peptide)

DFBP ID	DFBPSTPE0021
Peptide sequence	KAAVT
Type	Native peptide
Peptide/Function name	Stimulating peptide

Function-activity relationship

Main bioactivity	Stimulating activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Ala-Ala-Val-Thr

Single-letter amino acid

KAAVT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
488.3 Da	488.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

0.6400^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bos taurus (Bovine)
Precursor protein	Hemoglobin subunit beta
Residue position	f(7-11)
Precursor protein(s) search	Source.1: DFBPPR1424 ---- Plant proteins ---- Germin-like protein 8-14 Source.2: DFBPPR2257 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 11 Source.3: DFBPPR2399 ---- Plant proteins ---- Germin-like protein 5-1 Source.4: DFBPPR3884 ---- Plant proteins ---- Casparian strip membrane protein 4 Source.5: DFBPPR4929 ---- Plant proteins ---- DNA polymerase I A, chloroplastic Source.6: DFBPPR5494 ---- Plant proteins ---- Peroxidase 70 Source.7: DFBPPR16819 ---- Animal proteins ---- Hemoglobin subunit beta Source.8: DFBPPR18509 ---- Animal proteins ---- Hemoglobin fetal subunit beta Source.9: DFBPPR21587 ---- Animal proteins ---- Abnormal spindle-like microcephaly-associated protein homolog Source.10: DFBPPR10511 ---- Animal proteins ---- Vitellogenin-3 Source.11: DFBPPR10680 ---- Animal proteins ---- Hemoglobin subunit pi Source.12: DFBPPR11047 ---- Animal proteins ---- Nucleolin Source.13: DFBPPR13439 ---- Animal proteins ---- Hemoglobin subunit beta-A Source.14: DFBPPR13618 ---- Animal proteins ---- Hemoglobin subunit beta Source.15: DFBPPR13936 ---- Animal proteins ---- Abnormal spindle-like microcephaly-associated protein homolog Source.16: DFBPPR15178 ---- Microorganism protein ---- Phosphoglycerate kinase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Stimulating activity	Glucagon-Like Peptide 1 (GLP-1) enhancing capacity of the peptide was investigated. STC-1 cells were treated 2 h with each peptide tested at 0.1 and 1 mM. The peptide KAAVT induced the highest GLP-1 level when tested at 1 mM with 1264.2 ± 128.6 pM and was 32 times significantly higher than control level.
Specific target protein(s)	N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Bovine haemoglobin in vitro GI gigestion was performed by pepsin for 2 h, and then by pancreatin for 2 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Caron J, Cudennec B, Domenger D, Belguesmia Y, Flahaut C, Kouach M, Lesage J, Goossens JF, Dhulster P, Ravallec R. Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion. Food Res Int. 2016 Nov;89(Pt 1):382-390. PMID: 28460928 DOI: 10.1016/j.foodres.2016.08.033
Other literature(s)	N.D
PubDate	2016