Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPVACO0001(Vasoconstriction peptide)

DFBP ID	DFBPVACO0001
Peptide sequence	ETPDCFWKYCV
Type	Native peptide
Peptide/Function name	Vasoconstriction peptide, Urotensin II

Function-activity relationship

Main bioactivity	Vasoconstriction activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Thr-Pro-Asp-Cys-Phe-Trp-Lys-Tyr-Cys-Val

Single-letter amino acid

ETPDCFWKYCV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1390.6 Da	1390.60 Da ^c

Net charge

-1.1

Isoelectric point (pI)

4.1

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.3091^c

Hydrophilic residue ratio

36.36%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Fish
Organism/Source	Fish spinal cords
Precursor protein	Urotensin-II (U-II)
Residue position	N.D
Precursor protein(s) search	No matching precursor protein found
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Vasoconstriction activity

The potency of vasoconstriction of U-II is an order of magnitude greater than that of endothelin-1, making human U-II the most potent mammalian vasoconstrictor identified so far. Systemic administration is associated with profound cardiovascular collapse. As human U-II-like immunoreactivity is found within cardiac and vascular tissue (including coronary atheroma), this peptide may influence cardiovascular homeostasis and pathology (for example, in ischaemic heart disease and congestive heart failure).

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CS)C(=O)N[C@@]([H])(C(C)C)C(=O)O

Preparation method

Mode of preparation	Peptide synthesis
Enzyme(s)/starter culture	Human U-II peptidyl resin was synthesized (Milipore/Biosearch 9600 synthesizer), cleaved and de-protected before purification by reverse-phase HPLC. All other peptides were from Bachem.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Ames RS, Sarau HM, Chambers JK, Willette RN, Aiyar NV, Romanic AM, Louden CS, Foley JJ, Sauermelch CF, Coatney RW, Ao Z, Disa J, Holmes SD, Stadel JM, Martin JD, Liu WS, Glover GI, Wilson S, McNulty DE, Ellis CE, Elshourbagy NA, Shabon U, Trill JJ, Hay DW, Ohlstein EH, Bergsma DJ, Douglas SA. Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14. Nature. 1999 Sep 16;401(6750):282-6. PMID: 10499587 DOI: 10.1038/45809
Other literature(s)	[1] Bern, H. A., Pearson, D., Larson, B. A. & Nishioka, R. S. Neurohormones from fish tails: the caudal neurosecretory system. I. ''Urophysiology'' and the caudal neurosecretory system of fishes. Recent Prog. Horm. Res. 41, 533-552 (1985).
PubDate	1999