Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPVARE0004(Vasorelaxation peptide)

DFBP ID	DFBPVARE0004
Peptide sequence	RADHPF
Type	Native peptide
Peptide/Function name	Vasorelaxation peptide, Ovokinin (2-7)

Function-activity relationship

Main bioactivity	Vasorelaxation activity
Otheir bioactivity	Antihypertensive activity ^[D1], Antioxidative activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Arg-Ala-Asp-His-Pro-Phe

Single-letter amino acid

RADHPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	741.79 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

30 μM

pIC₅₀

-1.477

GRAVY

-1.3667^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg
Precursor protein	Ovalbumin (grade VI)
Residue position	f(359-364)
Precursor protein(s) search	Source.1: DFBPPR9993 ---- Animal proteins ---- Ovalbumin Source.2: DFBPPR10950 ---- Animal proteins ---- Ovalbumin-related protein Y Source.3: DFBPPR11500 ---- Animal proteins ---- Ovalbumin-related protein X
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

Vasorelaxation activity

(1) This peptide (30–300 μM) exerted a dose-dependent vasodilation in an isolated mesenteric artery from a spontaneously hypertensive rat which was pre-constricted by phenylephrine, besides the relaxation being endothelium-dependent.
(2) It is noteworthy that the nitric oxide synthase inhibitor N G-nitro-L-arginine methyl ester inhibited this relaxation, implying involvement of nitric oxide in its mechanism of action.
(3) Following oral administration of RADHPF at a dose of 10 mg/kg, the systolic blood pressure in a spontaneously hypertensive rat was significantly lowered.

Specific target protein(s)

N.D

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Ovalbumin (25 mg/ml) was adjusted to pH 7.8 with 0.5 N NaOH and digested two times by chymotrypsin (E/S = 1/100 (weight/weight)) for 12 h each at 37 ℃.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Ovokinin(2-7) could play an important role in the improvement of endothelial dysfunction as well as hypertension.

Database cross-references

DFBP

[D1]	DFBPANHY0333
[D2]	DFBPANOX0989
[D3]	DFBPMUFU0654

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Matoba, N.; Usui, H.; Fujita, H.; Yoshikawa, M., A novel anti-hypertensive peptide derived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Lett. 1999,452 (3), 181-184. DOI: 10.1016/S0014-5793(99)00587-6
Other literature(s)	N.D
PubDate	1999