Enzyme

Enzyme - 3.4.22.32

DFBP ID	DFBPENZY0041
EC Number	3.4.22.32
Term	Stem bromelain

Introduction

DFBP ID

DFBPENZY0041

EC Number

3.4.22.32

Accepted Name

Accepted name:
• Stem bromelain
Alternative name(s):
• Bromelain

Cleavage sites

Number	Pn------	P4	P3	P2	P1	Cleavage sites	P1'	P2'	------Pm'
site.1	------	-	-	-	V	-\|-	-	-	------
site.2	------	-	-	-	A	-\|-	-	-	------
site.3	------	-	-	-	T	-\|-	-	-	------
site.4	------	-	-	-	L	-\|-	-	-	------
site.5	------	-	-	-	R	-\|-	-	-	------
site.6	------	-	-	-	G	-\|-	-	-	------
site.7	------	-	-	-	S	-\|-	-	-	------
site.8	------	-	-	-	F	-\|-	-	-	------

Cross-references

Database(s)

ExPASy - ENZYME	EC 3.4.22.32
MEROPS database	Enzyme ID C01.005
ExplorEnz database	EC 3.4.22.32

Reference(s)

Literature(s)

[1] Brocklehurst, K., Willenbrock, F. and Salih, E. Cysteine proteinases. In: Neuberger, A. and Brocklehurst, K. (Ed.), New Comprehensive Biochemistry: Hydrolytic Enzymes, Elsevier, Amsterdam, 1987, pp. 39–158.
[2] Rowan, A.D., Buttle, D.J. and Barrett, A.J. Ananain: a novel cysteine proteinase found in pineapple stem. Arch. Biochem. Biophys. 267 (1988) 262–270. [DOI] [PMID: 3196029]
[3] Ritonja, A., Rowan, A.B., Buttle, D.J., Rawlings, N.D., Turk, V. and Barrett, A.J. Stem bromelain: amino acid sequence and implications for weak binding to cystatin. FEBS Lett. 247 (1989) 419–429. [DOI] [PMID: 2714443]
[4] Rowan, A.D., Buttle, D.J. and Barrett, A.J. The cysteine proteinases of the pineapple plant. Biochem. J. 266 (1990) 869–875. [PMID: 2327970]