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Enzyme - EC 3.4.21.62
DFBP ID DFBPENZY0061
EC Number EC 3.4.21.62
Term Subtilisin
Introduction
DFBP ID DFBPENZY0061
EC Number EC 3.4.21.62
Accepted Name

Accepted name:
     • Subtilisin

Cleavage sites
Number
Pn------
P4
P3
P2
P1
Cleavage sites
P1'
P2'
------Pm'
site.1------
-
--
Y
-|--
-
------
site.2
-------
-
-
F
-|--
-
------
site.3
-------
-
-
L
-|--
-
------
site.4-------
-
-
-
-|-V
-
------
site.5
-------
-
-
W
-|--
-
------
site.6-------
-
-
S
-|--
-
------
Cross-references
Database(s)
ExPASy - ENZYMEEC 3.4.21.62
MEROPS databaseEnzyme ID S08.034
ExplorEnz database
EC 3.4.21.62
Reference(s)
Literature(s)

[1] Ottesen, M. and Svendsen, I. The subtilisins. Methods Enzymol. 19 (1970) 199–215.
[2] Markland, F.S. and Smith, E.L. Subtilisins: primary structure, chemical and physical properties.  In: Boyer, P.D. (Ed.), The Enzymes, 3rd edn, vol. 3, Academic Press, New York, 1971, pp. 561–608.
[3] Philipp, M. and Bender, M.L. Kinetics of subtilisin and thiolsubtilisin. Mol. Cell. Biochem. 51 (1983) 5–32. [PMID: 6221910]
[4] Nedkov, P., Oberthür, W. and Braunitzer, G. Determination of the complete amino acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN, Carlsberg and amylosacchariticus. Biol. Chem. Hoppe-Seyler 366 (1985) 421–430. [PMID: 3927935]
[5] Ikemura, H., Takagi, H. and Inouye, M. Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli. J. Biol. Chem. 262 (1987) 7859–7864. [PMID: 3108260]
[6] Polgár, L. Structure and function of serine proteases.  In: Neuberger, A. and Brocklehurst, K. (Ed.), New Comprehensive Biochemistry: Hydrolytic Enzymes, vol. 16, Elsevier, Amsterdam, 1987, pp. 159–200.

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