AnOxP Prediction Pools
First, we use different enzymes (or combination enzymes) to simulate the hydrolysis of 21249 proteins from 60 species to obtain hydrolyzed fragments, and remove illegal sequences among them. Then, search the DFBP database to determine whether the obtained fragments are antioxidative peptides (AnOxPs), and screen out unreported sequences. Finally, use AnOxPP to predict these sequences, screen peptides with a certain probability range, and add them to the data pool.
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Enzyme(s) | Probability | Unique sequences | Prediction Pools | Download | |||||||
Combine and filter the fragments obtained from the following nine hydrolysis methods | |||||||||||
Reported by DFBP | 395 | reported_AnOxPs_all.txt | ![]() |
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No reported | 2321342 | unreported_fragments_all.txt | ![]() |
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< 0.50 | 548656 | probability_all < 0.50.txt | ![]() |
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≥ 0.50 | 1772686 | probability_all ≥ 0.50.txt | ![]() |
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≥ 0.90 | 1615584 | probability_all ≥ 0.90.txt | ![]() |
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≥ 0.95 | 1542585 | probability_all ≥ 0.95.txt | ![]() |
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Hydrolysis 1: | Chymotrypsin + Trypsin + Pepsin (pH=1.3) |
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Reported by DFBP | 133 | reported_AnOxPs_ChyTryPep.txt | ![]() |
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No reported | 313149 | unreported_fragments_ChyTryPep.txt | ![]() |
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< 0.50 | 32034 | probability_ChyTryPep < 0.50.txt | ![]() |
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≥ 0.50 | 281115 | probability_ChyTryPep ≥ 0.50.txt | ![]() |
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≥ 0.90 | 265412 | probability_ChyTryPep ≥ 0.90.txt | ![]() |
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≥ 0.95 | 256664 | probability_ChyTryPep ≥ 0.95.txt | ![]() |
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Hydrolysis 2: | Thermolysin + Papain |
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Reported by DFBP | 107 | reported_AnOxPs_ThePap.txt | ![]() |
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No reported | 154566 | unreported_fragments_ThePap.txt | ![]() |
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< 0.50 | 53069 | probability_ThePap < 0.50.txt | ![]() |
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≥ 0.50 | 101497 | probability_ThePap ≥ 0.50.txt | ![]() |
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≥ 0.90 | 83355 | probability_ThePap ≥ 0.90.txt | ![]() |
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≥ 0.95 | 74884 | probability_ThePap ≥ 0.95.txt | ![]() |
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Hydrolysis 3: | Pepsin + Pancreatic |
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Reported by DFBP | 95 | reported_AnOxPs_PepPan.txt | ![]() |
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No reported | 138379 | unreported_fragments_PepPan.txt | ![]() |
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< 0.50 | 76506 | probability_PepPan < 0.50.txt | ![]() |
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≥ 0.50 | 61873 | probability_PepPan ≥ 0.50.txt | ![]() |
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≥ 0.90 | 48460 | probability_PepPan ≥ 0.90.txt | ![]() |
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≥ 0.95 | 43292 | probability_PepPan ≥ 0.95.txt | ![]() |
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Hydrolysis 4: | Proteinase K | ||||||||||
Reported by DFBP | 67 | reported_AnOxPs_ProteinaseK.txt | ![]() |
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No reported | 188918 | unreported_fragments_ProteinaseK.txt | ![]() |
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< 0.50 | 76270 | probability_ProteinaseK < 0.50.txt | ![]() |
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≥ 0.50 | 112648 | probability_ProteinaseK ≥ 0.50.txt | ![]() |
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≥ 0.90 | 95463 | probability_ProteinaseK ≥ 0.90.txt | ![]() |
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≥ 0.95 | 88849 | probability_ProteinaseK ≥ 0.95.txt | ![]() |
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Hydrolysis 5: | Thermolysin | ||||||||||
Reported by DFBP | 113 | reported_AnOxPs_Thermolysin.txt | ![]() |
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No reported | 431224 | unreported_fragments_Thermolysin.txt | ![]() |
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< 0.50 | 92560 | probability_Thermolysin < 0.50.txt | ![]() |
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≥ 0.50 | 338664 | probability_Thermolysin ≥ 0.50.txt | ![]() |
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≥ 0.90 | 301105 | probability_Thermolysin ≥ 0.90.txt | ![]() |
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≥ 0.95 | 281660 | probability_Thermolysin ≥ 0.95.txt | ![]() |
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Hydrolysis 6: | Papain | ||||||||||
Reported by DFBP | 158 | reported_AnOxPs_Papain.txt | ![]() |
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No reported | 336030 | unreported_fragments_Papain.txt | ![]() |
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< 0.50 | 113717 | probability_Papain < 0.50.txt | ![]() |
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≥ 0.50 | 222313 | probability_Papain ≥ 0.50.txt | ![]() |
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≥ 0.90 | 195640 | probability_Papain ≥ 0.90.txt | ![]() |
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≥ 0.95 | 183137 | probability_Papain ≥ 0.95.txt | ![]() |
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Hydrolysis 7: | Pepsin (pH=1.3) | ||||||||||
Reported by DFBP | 268 | reported_AnOxPs_Pepsin.txt | ![]() |
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No reported | 437396 | unreported_fragments_Pepsin.txt | ![]() |
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< 0.50 | 112805 | probability_Pepsin < 0.50.txt | ![]() |
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≥ 0.50 | 324591 | probability_Pepsin ≥ 0.50.txt | ![]() |
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≥ 0.90 | 293809 | probability_Pepsin ≥ 0.90.txt | ![]() |
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≥ 0.95 | 279517 | probability_Pepsin ≥ 0.95.txt | ![]() |
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Hydrolysis 8: | Chymotrypsin | ||||||||||
Reported by DFBP | 129 | reported_AnOxPs_Chymotrypsin.txt | ![]() |
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No reported | 458355 | unreported_fragments_Chymotrypsin.txt | ![]() |
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< 0.50 | 73080 | probability_Chymotrypsin < 0.50.txt | ![]() |
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≥ 0.50 | 385275 | probability_Chymotrypsin ≥ 0.50.txt | ![]() |
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≥ 0.90 | 354989 | probability_Chymotrypsin ≥ 0.90.txt | ![]() |
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≥ 0.95 | 340481 | probability_Chymotrypsin ≥ 0.95.txt | ![]() |
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Hydrolysis 9: | Trypsin | ||||||||||
Reported by DFBP | 138 | reported_AnOxPs_Trypsin.txt | ![]() |
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No reported | 386104 | unreported_fragments_Trypsin.txt | ![]() |
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< 0.50 | 53050 | probability_Trypsin < 0.50.txt | ![]() |
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≥ 0.50 | 333054 | probability_Trypsin ≥ 0.50.txt | ![]() |
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≥ 0.90 | 321800 | probability_Trypsin ≥ 0.90.txt | ![]() |
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≥ 0.95 | 317554 | probability_Trypsin ≥ 0.95.txt | ![]() |