Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0003(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0003
Peptide sequence	IHPF
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ile-His-Pro-Phe

Single-letter amino acid

IHPF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	512.60 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

11.6 uM

pIC₅₀

-1.064

GRAVY

0.6250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	SHR plasma
Precursor protein	Angiotensin (ANG II: DRVYIHPF)
Residue position	f(5-8)
Precursor protein(s) search	Source.1: DFBPPR0921 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-5 Source.2: DFBPPR1507 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-4 Source.3: DFBPPR1577 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-6 Source.4: DFBPPR1913 ---- Plant proteins ---- Phosphoglucan, water dikinase, chloroplastic Source.5: DFBPPR2162 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-7 Source.6: DFBPPR2205 ---- Plant proteins ---- Cation transporter HKT1 Source.7: DFBPPR3122 ---- Plant proteins ---- Probable GTP diphosphokinase RSH2, chloroplastic Source.8: DFBPPR3288 ---- Plant proteins ---- Chitin-inducible gibberellin-responsive protein 2 Source.9: DFBPPR4267 ---- Plant proteins ---- Putative cyclin-D7-1 Source.10: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.11: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.12: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.13: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.14: DFBPPR16060 ---- Animal proteins ---- Protein kinase C delta type Source.15: DFBPPR18424 ---- Animal proteins ---- Inositol 1,4,5-trisphosphate receptor type 2 Source.16: DFBPPR22014 ---- Animal proteins ---- Solute carrier family 43 member 3 Source.17: DFBPPR9467 ---- Animal proteins ---- Metalloreductase STEAP1 Source.18: DFBPPR9998 ---- Animal proteins ---- Glycine dehydrogenase (decarboxylating), mitochondrial Source.19: DFBPPR13353 ---- Animal proteins ---- Angiotensinogen Source.20: DFBPPR13690 ---- Animal proteins ---- Angiotensinogen Source.21: DFBPPR14950 ---- Microorganism protein ---- 5'-AMP-activated protein kinase subunit gamma
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 11.6 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The peptide was extracted from the rat plasma.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	Non-Toxin
Prediction:	ToxinPred

Additional information

Although the active peptide is derived from rat serum, it can be obtained from food-borne proteins.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Matsufuji H, Matsui T, Ohshige S, Kawasaki T, Osajima K, Osajima Y. Antihypertensive effects of angiotensin fragments in SHR. Biosci Biotechnol Biochem. 1995 Aug;59(8):1398-401. PMID: 7549089 DOI: 10.1271/bbb.59.1398
Other literature(s)	[1] Matsui T, Matsufuji H, Kawasaki T, et al. Determination of endogenous peptides with in vitro ACE inhibitory activity in normotensive human plasma by the fluorometric HPLC method[J]. Bioscience Biotechnology (&) Biochemistry, 1997, 61(6):1052.
PubDate	1995