Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0036(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0036
Peptide sequence	HPGH
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

His-Pro-Gly-His

Single-letter amino acid

HPGH

Peptide length

Peptide mass

Experimental mass	Theoretical mass
428 Da	446.47 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.95 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-2.1000^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Defibrinated bovine plasma
Precursor protein	Bovine serum albumin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2693 ---- Plant proteins ---- Parkeol synthase Source.2: DFBPPR5080 ---- Plant proteins ---- Phytochrome A Source.3: DFBPPR7007 ---- Plant proteins ---- Protein Barley B recombinant Source.4: DFBPPR15940 ---- Animal proteins ---- Thyroid transcription factor 1 Source.5: DFBPPR17140 ---- Animal proteins ---- Adiponectin Source.6: DFBPPR17316 ---- Animal proteins ---- Forkhead box protein O1 Source.7: DFBPPR17353 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase-like N Source.8: DFBPPR17387 ---- Animal proteins ---- ATP-dependent DNA/RNA helicase DHX36 Source.9: DFBPPR17754 ---- Animal proteins ---- Amelogenin, X isoform Source.10: DFBPPR18399 ---- Animal proteins ---- Integrin beta-6 Source.11: DFBPPR18751 ---- Animal proteins ---- Mothers against decapentaplegic homolog 4 Source.12: DFBPPR21083 ---- Animal proteins ---- TRAF-interacting protein with FHA domain-containing protein A Source.13: DFBPPR21432 ---- Animal proteins ---- Amelogenin, Y isoform Source.14: DFBPPR22651 ---- Animal proteins ---- Spermatogenesis-associated protein 2-like protein Source.15: DFBPPR8601 ---- Animal proteins ---- Mothers against decapentaplegic homolog 4 Source.16: DFBPPR8741 ---- Animal proteins ---- Forkhead box protein O1 Source.17: DFBPPR9230 ---- Animal proteins ---- Transcription factor SOX-10 Source.18: DFBPPR10426 ---- Animal proteins ---- Transcription factor SOX-10 Source.19: DFBPPR10888 ---- Animal proteins ---- Nuclear distribution protein nudE-like 1 Source.20: DFBPPR11075 ---- Animal proteins ---- Sigma non-opioid intracellular receptor 1 Source.21: DFBPPR11175 ---- Animal proteins ---- Oligodendrocyte transcription factor 2 Source.22: DFBPPR11413 ---- Animal proteins ---- Tudor domain-containing protein 7 Source.23: DFBPPR11972 ---- Animal proteins ---- Cyclin-L1 Source.24: DFBPPR13078 ---- Animal proteins ---- Amelogenin-like protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was obtained form the peptide fractions with high ACE-inhibitory activity, so the peptide may have Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity, but IC₅₀ is not given.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC1=CN=C-N1)C(=O)N1[C@@]([H])(CCC1)C(=O)NCC(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with the microbial protease Flavourzyme L.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Bovine serum albumin is a potential source of ACE-inhibitory peptides in bovine plasma.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wanasundara PK, Ross AR, Amarowicz R, Ambrose SJ, Pegg RB, Shand PJ. Peptides with angiotensin I-converting enzyme (ACE) inhibitory activity from defibrinated, hydrolyzed bovine plasma. J Agric Food Chem. 2002 Nov 20;50(24):6981-8. PMID: 12428947 DOI: 10.1021/jf025592e
Other literature(s)	N.D
PubDate	2002