Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0071(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0071
Peptide sequence	YGGVSLPEW
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Gly-Gly-Val-Ser-Leu-Pro-Glu-Trp

Single-letter amino acid

YGGVSLPEW

Peptide length

Peptide mass

Experimental mass	Theoretical mass
1007 Da	1007.11 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

1 uM

pIC₅₀

0.0

GRAVY

-0.1000^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	α-Lactalbumin
Residue position	f(18-26)
Precursor protein(s) search	Source.1: DFBPPR7685 ---- Milk proteins ---- Alpha-lactalbumin Source.2: DFBPPR7697 ---- Milk proteins ---- Alpha-lactalbumin Source.3: DFBPPR7711 ---- Milk proteins ---- Alpha-lactalbumin Source.4: DFBPPR8490 ---- Milk proteins ---- Alpha-lactalbumin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 1 μM (48% ACE inhibition).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The thermolysin catalysed hydrolysates of α-lactalbumin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANOX0614
[D2]	DFBPMUFU0033

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Otte, J., Shalaby, S.M.A., Zakora, M., Nielsen, M.S. Fractionation and identification of ACE-inhibitory peptides from α-lactalbumin and β-casein produced by thermolysin-catalysed hydrolysis. International Dairy Journal. 2007, 17, 1460-72. DOI: 10.1016/j.idairyj.2007.04.008
Other literature(s)	N.D
PubDate	2007