Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0112(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0112
Peptide sequence	GFHI
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antimicrobial activity ^[D1], Anticancer activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Gly-Phe-His-Ile

Single-letter amino acid

GFHI

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	472.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

117 ug/ml

pIC₅₀

-2.068

GRAVY

0.9250^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Beef sarcoplasmic
Precursor protein	Beef sarcoplasmic protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0863 ---- Plant proteins ---- Brassinosteroid LRR receptor kinase BRI1 Source.2: DFBPPR1352 ---- Plant proteins ---- Superoxide dismutase [Cu-Zn] 1 Source.3: DFBPPR1892 ---- Plant proteins ---- Sucrose synthase 1 Source.4: DFBPPR2046 ---- Plant proteins ---- Double-strand break repair protein MRE11 Source.5: DFBPPR2057 ---- Plant proteins ---- Cytochrome P450 87A3 Source.6: DFBPPR3155 ---- Plant proteins ---- Acyl transferase 1 Source.7: DFBPPR3935 ---- Plant proteins ---- Sucrose synthase 4 Source.8: DFBPPR4335 ---- Plant proteins ---- Actin-related protein 5 Source.9: DFBPPR4380 ---- Plant proteins ---- Sucrose synthase 6 Source.10: DFBPPR5056 ---- Plant proteins ---- Sucrose synthase Source.11: DFBPPR5076 ---- Plant proteins ---- Xyloglucan endotransglucosylase/hydrolase 1 Source.12: DFBPPR5707 ---- Plant proteins ---- indole-2-monooxygenase Source.13: DFBPPR5985 ---- Plant proteins ---- Aquaporin TIP4-3 Source.14: DFBPPR6322 ---- Plant proteins ---- Superoxide dismutase [Cu-Zn] Source.15: DFBPPR6506 ---- Plant proteins ---- Sucrose synthase 2 Source.16: DFBPPR6659 ---- Plant proteins ---- Glucose-1-phosphate adenylyltransferase large subunit Source.17: DFBPPR7536 ---- Plant proteins ---- 60S ribosomal protein L16, mitochondrial Source.18: DFBPPR8197 ---- Plant proteins ---- Aconitate hydratase, cytoplasmic Source.19: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.20: DFBPPR18420 ---- Animal proteins ---- Cytochrome P450 2D14 Source.21: DFBPPR18871 ---- Animal proteins ---- Methionine synthase Source.22: DFBPPR19612 ---- Animal proteins ---- Lysosomal-trafficking regulator Source.23: DFBPPR19958 ---- Animal proteins ---- 60S ribosomal protein L10 Source.24: DFBPPR20329 ---- Animal proteins ---- Meiosis regulator and mRNA stability factor 1 Source.25: DFBPPR22065 ---- Animal proteins ---- 60S ribosomal protein L10-like Source.26: DFBPPR22147 ---- Animal proteins ---- Immunoglobulin superfamily containing leucine-rich repeat protein Source.27: DFBPPR22750 ---- Animal proteins ---- Uncharacterized protein C4orf45 homolog Source.28: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.29: DFBPPR9294 ---- Animal proteins ---- 60S ribosomal protein L10 Source.30: DFBPPR9954 ---- Animal proteins ---- Tolloid-like protein 1 Source.31: DFBPPR10192 ---- Animal proteins ---- Vascular endothelial growth factor receptor 1 Source.32: DFBPPR10296 ---- Animal proteins ---- Mucin-5B Source.33: DFBPPR10337 ---- Animal proteins ---- Alpha-tectorin Source.34: DFBPPR10395 ---- Animal proteins ---- X-ray repair cross-complementing protein 5 Source.35: DFBPPR10532 ---- Animal proteins ---- Carnosine N-methyltransferase Source.36: DFBPPR11357 ---- Animal proteins ---- Fibroblast growth factor 4 Source.37: DFBPPR11480 ---- Animal proteins ---- Cytochrome P450 1A2 Source.38: DFBPPR11655 ---- Animal proteins ---- 60S ribosomal protein L10 Source.39: DFBPPR12254 ---- Animal proteins ---- Cytochrome P450 1A2 Source.40: DFBPPR13843 ---- Animal proteins ---- 60S ribosomal protein L10 Source.41: DFBPPR14977 ---- Microorganism protein ---- Superoxide dismutase [Cu-Zn] Source.42: DFBPPR7964 ---- Plant protein ---- Sucrose synthase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 117 ug/ml.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with commercial enzymes (proteinase k).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The ACE inhibitory peptides can be expected to possess other biological functions and used as functional materials for food industry.

Database cross-references

DFBP

[D1]	DFBPAMIC0515
[D2]	DFBPANCA0330
[D3]	DFBPMUFU0046

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Jang, A., Jo, C., Kang, K.-S., Lee, M. Antimicrobial and human cancer cell cytotoxic effect of synthetic angiotensin-converting enzyme (ACE) inhibitory peptides. Food Chem. 2008, 107, 327-36. DOI: 10.1016/j.foodchem.2007.08.036
Other literature(s)	N.D
PubDate	2008