Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0115(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0115
Peptide sequence	GLSDGEWQ
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antimicrobial activity ^[D1], Anticancer activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Gly-Leu-Ser-Asp-Gly-Glu-Trp-Gln

Single-letter amino acid

GLSDGEWQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	890.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.04 ^c

IC₅₀

50.5 ug/ml

pIC₅₀

-1.703

GRAVY

-1.1500^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Beef sarcoplasmic
Precursor protein	Beef sarcoplasmic protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR16534 ---- Animal proteins ---- Myoglobin Source.2: DFBPPR18268 ---- Animal proteins ---- Myoglobin Source.3: DFBPPR9497 ---- Animal proteins ---- Myoglobin Source.4: DFBPPR13323 ---- Animal proteins ---- Myoglobin Source.5: DFBPPR13812 ---- Animal proteins ---- Myoglobin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 50.5 ug/ml.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(=O)O)C(=O)NCC(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=CN2)C1=C2C=CC=C1)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with commercial enzymes (thermolysin+proteinase A).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The ACE inhibitory peptides can be expected to possess other biological functions and used as functional materials for food industry.

Database cross-references

DFBP

[D1]	DFBPAMIC0514
[D2]	DFBPANCA0332
[D3]	DFBPMUFU0049

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Jang, A., Jo, C., Kang, K.-S., Lee, M. Antimicrobial and human cancer cell cytotoxic effect of synthetic angiotensin-converting enzyme (ACE) inhibitory peptides. Food Chem. 2008, 107, 327-36. DOI: 10.1016/j.foodchem.2007.08.036
Other literature(s)	N.D
PubDate	2008