Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0119(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0119
Peptide sequence	AQSAP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ala-Gln-Ser-Ala-Pro

Single-letter amino acid

AQSAP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	472.49 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

About 238.6 ± 49.20 ug/ml (Pre-peptide activity)

pIC₅₀

About 238.6 ± 49.20 ug/ml (Pre-peptide activity)

GRAVY

-0.4600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk proteins
Precursor protein	β-Lactoglobulin
Residue position	f(34-38)
Precursor protein(s) search	Source.1: DFBPPR0115 ---- Milk proteins ---- Beta-lactoglobulin Source.2: DFBPPR4035 ---- Plant proteins ---- Probable transcription factor MYB58 Source.3: DFBPPR7687 ---- Milk proteins ---- Beta-lactoglobulin Source.4: DFBPPR7698 ---- Milk proteins ---- Beta-lactoglobulin-1/B Source.5: DFBPPR7714 ---- Milk proteins ---- Beta-lactoglobulin Source.6: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.7: DFBPPR17131 ---- Animal proteins ---- Hepatocyte growth factor receptor Source.8: DFBPPR17802 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.9: DFBPPR18383 ---- Animal proteins ---- Transcription factor E3 Source.10: DFBPPR19752 ---- Animal proteins ---- Chromatin target of PRMT1 protein Source.11: DFBPPR20333 ---- Animal proteins ---- RNA-binding protein 14 Source.12: DFBPPR12052 ---- Animal proteins ---- Testis-expressed protein 10 homolog Source.13: DFBPPR12317 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP4 Source.14: DFBPPR13585 ---- Animal proteins ---- Hepatocyte growth factor receptor
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of about 238.6 ± 49.20 ug/ml (19.2 ± 4.0% ACE inhibition, Pre-peptide activity).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Fermented with Lb. helveticus.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The peptide may have antihypertensive effect as natural and safe nutraceutical/functional ingredients.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Ahn, J.E., Park, S.Y., Atwal, A., Gibbs, B.F., Lee, B.H. Angiotensin I-Converting Enzyme (Ace) Inhibitory Peptides from Whey Fermented by Lactobacillus Species. Journal of Food Biochemistry. 2009, 33, 587-602. DOI: 10.1111/j.1745-4514.2009.00239.x
Other literature(s)	N.D
PubDate	2009