Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0159(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0159
Peptide sequence	RVPSL
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Antithrombotic activity ^[D2], α-Amylase inhibitory activity ^[D3], α-Glucosidase inhibitory activity ^[D4], Multifunctional activity ^[D5]

Calculated physicochemical properties

Three-letter amino acid

Arg-Val-Pro-Ser-Leu

Single-letter amino acid

RVPSL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	570.68 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

20 uM

pIC₅₀

-1.301

GRAVY

0.2200^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Egg white protein
Precursor protein	Ovotransferrin
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.2: DFBPPR1866 ---- Plant proteins ---- Probable DNA gyrase subunit A, chloroplastic/mitochondrial Source.3: DFBPPR19041 ---- Animal proteins ---- Presenilins-associated rhomboid-like protein, mitochondrial Source.4: DFBPPR9971 ---- Animal proteins ---- Ovotransferrin Source.5: DFBPPR12059 ---- Animal proteins ---- Solute carrier family 46 member 3
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 20 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with alkaline proteinase Alcalase.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) The peptides RVPSL, in a range of 5–50 mg kg−1, exerted an anxiolytic effect on the SHRs^[1].
(2) The egg white derived peptides RVPSL could be considered as possible functional food or food ingredients due to their in vivo anti-stress and anti-anxiety effects on the SHRs ^[1].

Database cross-references

DFBP

[D1]	DFBPANHY0168, DFBPANHY0287
[D2]	DFBPANTH0093
[D3]	DFBPALAM0016
[D4]	DFBPALGL0007
[D5]	DFBPMUFU0062

[D6]

[D7]

[D8]

[D9]

Reference(s)

Primary literature	Yu Z, Liu B, Zhao W, Yin Y, Liu J, Chen F. Primary and secondary structure of novel ACE-inhibitory peptides from egg white protein. Food Chem. 2012 Jul 15;133(2):315-22. PMID: 25683401 DOI: 10.1016/j.foodchem.2012.01.032
Other literature(s)	[1] Yu Z, Zhao W, Ding L, et al. Anxiolytic effects of ACE inhibitory peptides on the behavior of rats in an elevated plus-maze[J]. Food (&) Function, 2015, 7(1):491-497. [2] Liu J B, Yu Z P, Zhao W Z, et al. Isolation and identification of angiotensin-converting enzyme inhibitory peptides from egg white protein hydrolysates[J]. Food Chemistry, 2010, 122(4):1159-1163.
PubDate	2012