Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0358(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0358
Peptide sequence	ALPMH
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide, β-Lactokinin

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Hypocholesterolemic activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Leu-Pro-Met-His

Single-letter amino acid

ALPMH

Peptide length

Peptide mass

Experimental mass	Theoretical mass
568.3 Da	567.70 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

7.79 ^c

IC₅₀

521 uM

pIC₅₀

-2.717

GRAVY

0.5400^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk whey protein
Precursor protein	β-Lactoglobulin
Residue position	f(142-146)
Precursor protein(s) search	Source.1: DFBPPR0115 ---- Milk proteins ---- Beta-lactoglobulin Source.2: DFBPPR7687 ---- Milk proteins ---- Beta-lactoglobulin Source.3: DFBPPR7698 ---- Milk proteins ---- Beta-lactoglobulin-1/B Source.4: DFBPPR7714 ---- Milk proteins ---- Beta-lactoglobulin Source.5: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.6: DFBPPR19381 ---- Animal proteins ---- BRCA2 and CDKN1A-interacting protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited low Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 521 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCSC)C(=O)N[C@@]([H])(CC1=CN=C-N1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The bovine whey protein β-lactoglobulin was hydrolysed with pepsin, then trypsin and chymotrypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	No measured
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPHYCP0003
[D2]	DFBPMUFU0404

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Pihlanto-Leppälä A, Koskinen P, Piilola K, Tupasela T, Korhonen H. Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides. J Dairy Res. 2000 Feb;67(1):53-64. PMID: 10717843 DOI: 10.1017/S0022029999003982
Other literature(s)	[1] Bösze Z. Bioactive Components of Milk[M]. Springer New York, 2008. [2] Mullally M M, Meisel H, Fitzgerald R J. Identification of a novel angiotensin‐I‐converting enzyme inhibitory peptide corresponding to a tryptic fragment of bovine β‐lactoglobulin[J]. Febs Letters, 1997, 402(2-3):99–101.
PubDate	2000