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List of peptide properties
DFBP ID - DFBPACEI0384(ACE-inhibitory peptide)
DFBP ID DFBPACEI0384
Peptide sequence LIYP
Type Native peptide
Peptide/Function name ACE-inhibitory peptide
Function-activity relationship
Main bioactivity ACE-inhibitory activity
Otheir bioactivity PEP-inhibitory activity [D1], Multifunctional activity [D2]
Calculated physicochemical properties
Three-letter amino acid Leu-Ile-Tyr-Pro
Single-letter amino acid LIYP
Peptide length 4
Peptide mass
Experimental mass Theoretical mass
N.D 504.61 Da c
Net charge 0.00 c
Isoelectric point (pI) 5.92 c
IC50

10 μM

pIC50 -1.0
GRAVY 1.3500 c
Hydrophilic residue ratio 75% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Human
Organism/Source Human milk protein
Precursor protein β-Casein
Residue position

f(49-52)

Precursor protein(s) search
Source.1: DFBPPR1069 ---- Plant proteins ---- Cinnamoyl-CoA reductase 1
Source.2: DFBPPR1072 ---- Plant proteins ---- Cytokinin dehydrogenase 2
Source.3: DFBPPR1161 ---- Plant proteins ---- Photosystem II protein D1
Source.4: DFBPPR1652 ---- Plant proteins ---- Photosystem II D2 protein
Source.5: DFBPPR1872 ---- Plant proteins ---- Cytokinin dehydrogenase 5
Source.6: DFBPPR1888 ---- Plant proteins ---- Cytokinin dehydrogenase 11
Source.7: DFBPPR1925 ---- Plant proteins ---- Cytokinin dehydrogenase 3
Source.8: DFBPPR2171 ---- Plant proteins ---- Probable mixed-linked glucan synthase 8
Source.9: DFBPPR3910 ---- Plant proteins ---- Probable potassium transporter 3
Source.10: DFBPPR4063 ---- Plant proteins ---- Cleavage and polyadenylation specificity factor subunit 2
Source.11: DFBPPR4239 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL10
Source.12: DFBPPR4447 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL1
Source.13: DFBPPR4993 ---- Plant proteins ---- Photosystem II protein D1
Source.14: DFBPPR5063 ---- Plant proteins ---- Photosystem II D2 protein
Source.15: DFBPPR5245 ---- Plant proteins ---- Omega-6 fatty acid desaturase, chloroplastic
Source.16: DFBPPR5394 ---- Plant proteins ---- Photosystem II protein D1
Source.17: DFBPPR5477 ---- Plant proteins ---- Photosystem II D2 protein
Source.18: DFBPPR5915 ---- Plant proteins ---- Homocysteine S-methyltransferase 2
Source.19: DFBPPR5916 ---- Plant proteins ---- Homocysteine S-methyltransferase 3
Source.20: DFBPPR6220 ---- Plant proteins ---- Photosystem II protein D1
Source.21: DFBPPR6232 ---- Plant proteins ---- Photosystem II D2 protein
Source.22: DFBPPR6648 ---- Plant proteins ---- Photosystem II protein D1
Source.23: DFBPPR6710 ---- Plant proteins ---- Photosystem II D2 protein
Source.24: DFBPPR7023 ---- Plant proteins ---- Photosystem II protein D1
Source.25: DFBPPR7054 ---- Plant proteins ---- Photosystem II D2 protein
Source.26: DFBPPR7205 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta''
Source.27: DFBPPR7401 ---- Plant proteins ---- Photosystem II protein D1
Source.28: DFBPPR7605 ---- Milk proteins ---- Beta-casein
Source.29: DFBPPR8452 ---- Plant proteins ---- Photosystem II protein D1
Source.30: DFBPPR8453 ---- Plant proteins ---- Photosystem II D2 protein
Source.31: DFBPPR16567 ---- Animal proteins ---- Oxygen-regulated protein 1
Source.32: DFBPPR16671 ---- Animal proteins ---- Forkhead box protein I3
Source.33: DFBPPR17766 ---- Animal proteins ---- Acetylcholine receptor subunit delta
Source.34: DFBPPR17903 ---- Animal proteins ---- Transcription initiation factor IIB
Source.35: DFBPPR18328 ---- Animal proteins ---- Delta-aminolevulinic acid dehydratase
Source.36: DFBPPR18347 ---- Animal proteins ---- Nucleolar complex protein 2 homolog
Source.37: DFBPPR18422 ---- Animal proteins ---- Endonuclease III-like protein 1
Source.38: DFBPPR18498 ---- Animal proteins ---- Neutral cholesterol ester hydrolase 1
Source.39: DFBPPR18777 ---- Animal proteins ---- Interleukin-1 receptor-associated kinase-like 2
Source.40: DFBPPR18819 ---- Animal proteins ---- Proteasome activator complex subunit 4
Source.41: DFBPPR19172 ---- Animal proteins ---- Persulfide dioxygenase ETHE1, mitochondrial
Source.42: DFBPPR19404 ---- Animal proteins ---- Microfibril-associated glycoprotein 4
Source.43: DFBPPR19474 ---- Animal proteins ---- Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Source.44: DFBPPR20041 ---- Animal proteins ---- Arylacetamide deacetylase
Source.45: DFBPPR20405 ---- Animal proteins ---- Oxygen-regulated protein 1
Source.46: DFBPPR20492 ---- Animal proteins ---- Microtubule-associated protein 10
Source.47: DFBPPR20543 ---- Animal proteins ---- Procollagen galactosyltransferase 1
Source.48: DFBPPR21002 ---- Animal proteins ---- Olfactomedin-like protein 2B
Source.49: DFBPPR9220 ---- Animal proteins ---- Alpha-1D adrenergic receptor
Source.50: DFBPPR11574 ---- Animal proteins ---- LHFPL tetraspan subfamily member 5 protein
Source.51: DFBPPR11971 ---- Animal proteins ---- Protein YIPF4
Source.52: DFBPPR12152 ---- Animal proteins ---- Asparagine synthetase domain-containing protein 1
Source.53: DFBPPR12421 ---- Animal proteins ---- Arylacetamide deacetylase
Source.54: DFBPPR12840 ---- Animal proteins ---- Alpha-1D adrenergic receptor
Source.55: DFBPPR14291 ---- Marine protein ---- Photosystem II D2 protein
Source.56: DFBPPR14664 ---- Marine protein ---- NADH-ubiquinone oxidoreductase chain 5
Source.57: DFBPPR15164 ---- Microorganism protein ---- Methionine aminopeptidase 2
Source.58: DFBPPR7757 ---- Plant protein ---- Photosystem II protein D1
Source.59: DFBPPR7775 ---- Plant protein ---- Photosystem II D2 protein
Source.60: DFBPPR7929 ---- Plant protein ---- Photosystem II protein D1
Source.61: DFBPPR8031 ---- Plant protein ---- Photosystem II protein D1
Source.62: DFBPPR8048 ---- Plant protein ---- NADP-dependent malic enzyme
Source.63: DFBPPR8050 ---- Plant protein ---- Photosystem II D2 protein
Source.64: DFBPPR8216 ---- Plant protein ---- Photosystem II protein D1
Source.65: DFBPPR8226 ---- Plant protein ---- Photosystem II D2 protein
Link-research
There are no literature reports on the discovery of this sequence in other food-source proteins.
Biological/Functional activity & target protein
ACE-inhibitory activity

The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC50 value of 10 uM.

Specific target protein(s) Specific Target Protein(s):
Angiotensin-converting enzyme
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)O
Preparation method
Mode of preparation

Synthesis

Enzyme(s)/starter culture

The peptides used in this study were synthesized by the solid-phase method.

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information N.D
Database cross-references
DFBP
[D1] DFBPPEPI0054
[D2] DFBPMUFU0408
BIOPEP-UWM [D3] 2628
APD [D4] -
BioPepDB [D5] -
MBPDB [D6] -
Reference(s)
Primary literature Kohmura, M., Nio, N., Kubo, K., Minoshima, Y., Munekata, E., Ariyoshi, Y. Inhibition of Angiotensin-converting Enzyme by Synthetic Peptides of Human β-Casein. Agricultural and Biological Chemistry. 2014, 53, 2107-14.
Other literature(s) N.D
PubDate 2014
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214