Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0385(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0385
Peptide sequence	PLIYP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], PEP-inhibitory activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Pro-Leu-Ile-Tyr-Pro

Single-letter amino acid

PLIYP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	601.73 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

4.4 μM

pIC₅₀

-0.643

GRAVY

0.7600^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Human
Organism/Source	Human milk protein
Precursor protein	β-Casein
Residue position	f(48-52)
Precursor protein(s) search	Source.1: DFBPPR1069 ---- Plant proteins ---- Cinnamoyl-CoA reductase 1 Source.2: DFBPPR5245 ---- Plant proteins ---- Omega-6 fatty acid desaturase, chloroplastic Source.3: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR18347 ---- Animal proteins ---- Nucleolar complex protein 2 homolog Source.5: DFBPPR9220 ---- Animal proteins ---- Alpha-1D adrenergic receptor Source.6: DFBPPR12840 ---- Animal proteins ---- Alpha-1D adrenergic receptor
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 4.4 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptides used in this study were synthesized by the conventional liquid-phase method.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPANHY0915
[D2]	DFBPPEPI0055
[D3]	DFBPMUFU0409

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Kohmura, M., Nio, N., Kubo, K., Minoshima, Y., Munekata, E., Ariyoshi, Y. Inhibition of Angiotensin-converting Enzyme by Synthetic Peptides of Human β-Casein. Agricultural and Biological Chemistry. 2014, 53, 2107-14. DOI: 10.1080/00021369.1989.10869621
Other literature(s)	N.D
PubDate	2014