Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0672(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0672
Peptide sequence	TQVY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Thr-Gln-Val-Tyr

Single-letter amino acid

TQVY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	509.56 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

18.2 μM

pIC₅₀

-1.26

GRAVY

-0.3250^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Rice
Precursor protein	Seed protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1763 ---- Plant proteins ---- E3 ubiquitin-protein ligase SRFP1 Source.2: DFBPPR2845 ---- Plant proteins ---- ABC transporter G family member 47 Source.3: DFBPPR2846 ---- Plant proteins ---- ABC transporter G family member 46 Source.4: DFBPPR3259 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP2A-3 catalytic subunit Source.5: DFBPPR3614 ---- Plant proteins ---- Prolamin PPROL 17D Source.6: DFBPPR3833 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP2A-1 catalytic subunit Source.7: DFBPPR3922 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP2A-4 catalytic subunit Source.8: DFBPPR3933 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP2A-2 catalytic subunit Source.9: DFBPPR3992 ---- Plant proteins ---- Probable uridine nucleosidase 2 Source.10: DFBPPR5458 ---- Plant proteins ---- Ribulose bisphosphate carboxylase small chain, chloroplastic Source.11: DFBPPR6307 ---- Plant proteins ---- Phytochrome-associated serine/threonine-protein phosphatase Source.12: DFBPPR7528 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP2A catalytic subunit Source.13: DFBPPR16607 ---- Animal proteins ---- Taste receptor type 1 member 2 Source.14: DFBPPR17073 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Source.15: DFBPPR17137 ---- Animal proteins ---- Interleukin-1 receptor-associated kinase 1 Source.16: DFBPPR17800 ---- Animal proteins ---- E3 ubiquitin-protein ligase RNF13 Source.17: DFBPPR17919 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Source.18: DFBPPR18315 ---- Animal proteins ---- Serine/threonine-protein phosphatase 4 catalytic subunit Source.19: DFBPPR18350 ---- Animal proteins ---- BRISC and BRCA1-A complex member 2 Source.20: DFBPPR18819 ---- Animal proteins ---- Proteasome activator complex subunit 4 Source.21: DFBPPR19798 ---- Animal proteins ---- Uricase Source.22: DFBPPR20265 ---- Animal proteins ---- Histone-lysine N-methyltransferase EZH1 Source.23: DFBPPR20435 ---- Animal proteins ---- Leucine-rich glioma-inactivated protein 1 Source.24: DFBPPR20853 ---- Animal proteins ---- Hemopexin Source.25: DFBPPR21384 ---- Animal proteins ---- Transcription factor Sp2 Source.26: DFBPPR22542 ---- Animal proteins ---- Transmembrane protein 151B Source.27: DFBPPR9093 ---- Animal proteins ---- Hemopexin Source.28: DFBPPR9166 ---- Animal proteins ---- Uricase Source.29: DFBPPR9190 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Source.30: DFBPPR10354 ---- Animal proteins ---- DNA topoisomerase 2-beta Source.31: DFBPPR10433 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Source.32: DFBPPR12534 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Source.33: DFBPPR12594 ---- Animal proteins ---- Serine/threonine-protein phosphatase 4 catalytic subunit Source.34: DFBPPR12629 ---- Animal proteins ---- Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Source.35: DFBPPR12725 ---- Animal proteins ---- Uricase Source.36: DFBPPR15515 ---- Microorganism protein ---- Tethering factor for nuclear proteasome STS1 Source.37: DFBPPR15623 ---- Microorganism protein ---- General transcriptional corepressor TUP1 Source.38: DFBPPR8319 ---- Plant protein ---- Serine/threonine-protein phosphatase PP2A catalytic subunit
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 18.2 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Rice protein was hydrolyzed by protease Alcalase for 2 h at 55 ℃ (Alcalase 2.4 L is a food-grade endoprotease from Bacillus licheniformis and the main enzyme component is the serine protease subtilisin A, EC3.4.21.62).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The rice protein hydrolysate prepared with Alcalase might be utilized to develop physiologically functional food with antihypertensive activity.

Database cross-references

DFBP

[D1]	DFBPANHY0306
[D2]	DFBPMUFU0151

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Li GH, Qu MR, Wan JZ, You JM. Antihypertensive effect of rice protein hydrolysate with in vitro angiotensin I-converting enzyme inhibitory activity in spontaneously hypertensive rats. Asia Pac J Clin Nutr. 2007;16 Suppl 1:275-80. PMID: 17392118
Other literature(s)	N.D
PubDate	2007