Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0709(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0709
Peptide sequence	IYLL
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ile-Tyr-Leu-Leu

Single-letter amino acid

IYLL

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	520.65 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

42 mM

pIC₅₀

N.D

GRAVY

2.7000^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soy
Precursor protein	Soybean protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0924 ---- Plant proteins ---- Two pore potassium channel a Source.2: DFBPPR1147 ---- Plant proteins ---- ABC transporter C family member 13 Source.3: DFBPPR1638 ---- Plant proteins ---- 3-hydroxy-3-methylglutaryl-coenzyme A reductase 3 Source.4: DFBPPR1846 ---- Plant proteins ---- Cytokinin dehydrogenase 1 Source.5: DFBPPR1856 ---- Plant proteins ---- Aminopeptidase M1-D Source.6: DFBPPR2089 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 3, mitochondrial Source.7: DFBPPR2355 ---- Plant proteins ---- Probable glycosyltransferase 5 Source.8: DFBPPR2668 ---- Plant proteins ---- Two-component response regulator ORR33 Source.9: DFBPPR2770 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic Source.10: DFBPPR2780 ---- Plant proteins ---- Coatomer subunit gamma-2 Source.11: DFBPPR2789 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.12: DFBPPR2921 ---- Plant proteins ---- Mannan endo-1,4-beta-mannosidase 3 Source.13: DFBPPR2922 ---- Plant proteins ---- Probable glycosyltransferase 2 Source.14: DFBPPR3046 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 35B Source.15: DFBPPR3240 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 35A Source.16: DFBPPR3399 ---- Plant proteins ---- Mannan endo-1,4-beta-mannosidase 4 Source.17: DFBPPR3445 ---- Plant proteins ---- Serine/threonine-protein kinase ATR Source.18: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.19: DFBPPR3610 ---- Plant proteins ---- Auxin transporter-like protein 1 Source.20: DFBPPR3715 ---- Plant proteins ---- Auxin transporter-like protein 3 Source.21: DFBPPR4281 ---- Plant proteins ---- Probable mannose-1-phosphate guanylyltransferase 3 Source.22: DFBPPR4375 ---- Plant proteins ---- Magnesium transporter MRS2-E Source.23: DFBPPR4397 ---- Plant proteins ---- Two-component response regulator ORR31 Source.24: DFBPPR4404 ---- Plant proteins ---- Two-component response regulator ORR32 Source.25: DFBPPR4467 ---- Plant proteins ---- Probable mannose-1-phosphate guanylyltransferase 1 Source.26: DFBPPR4470 ---- Plant proteins ---- Probable mannose-1-phosphate guanylyltransferase 2 Source.27: DFBPPR4571 ---- Plant proteins ---- CASP-like protein 1D1 Source.28: DFBPPR4581 ---- Plant proteins ---- LIMR family protein Os06g0128200 Source.29: DFBPPR5124 ---- Plant proteins ---- Nodulin-26 Source.30: DFBPPR5174 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.31: DFBPPR5522 ---- Plant proteins ---- ABC transporter C family MRP4 Source.32: DFBPPR5628 ---- Plant proteins ---- 3-hydroxy-3-methylglutaryl-coenzyme A reductase Source.33: DFBPPR5768 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic Source.34: DFBPPR5814 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.35: DFBPPR5992 ---- Plant proteins ---- Aquaporin NIP3-1 Source.36: DFBPPR6047 ---- Plant proteins ---- CASP-like protein 1D1 Source.37: DFBPPR6822 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic Source.38: DFBPPR6831 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.39: DFBPPR7122 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic Source.40: DFBPPR7187 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.41: DFBPPR7527 ---- Plant proteins ---- Serine/threonine-protein phosphatase PP1 Source.42: DFBPPR8430 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.43: DFBPPR15961 ---- Animal proteins ---- C-C chemokine receptor type 5 Source.44: DFBPPR16297 ---- Animal proteins ---- Nuclear factor NF-kappa-B p105 subunit Source.45: DFBPPR16479 ---- Animal proteins ---- Carboxypeptidase B Source.46: DFBPPR16871 ---- Animal proteins ---- Plasminogen Source.47: DFBPPR17029 ---- Animal proteins ---- Cytochrome b-245 light chain Source.48: DFBPPR17085 ---- Animal proteins ---- Cadherin-2 Source.49: DFBPPR17166 ---- Animal proteins ---- Cyclic nucleotide-gated cation channel beta-1 Source.50: DFBPPR17194 ---- Animal proteins ---- Aspartate aminotransferase, cytoplasmic Source.51: DFBPPR17501 ---- Animal proteins ---- C-C chemokine receptor type 5 Source.52: DFBPPR17843 ---- Animal proteins ---- Vacuolar protein sorting-associated protein 33B Source.53: DFBPPR18000 ---- Animal proteins ---- Very-long-chain enoyl-CoA reductase Source.54: DFBPPR18002 ---- Animal proteins ---- Toll-like receptor 10 Source.55: DFBPPR18210 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.56: DFBPPR18385 ---- Animal proteins ---- CTD nuclear envelope phosphatase 1 Source.57: DFBPPR18843 ---- Animal proteins ---- Carboxypeptidase B Source.58: DFBPPR19082 ---- Animal proteins ---- Protein SCO2 homolog, mitochondrial Source.59: DFBPPR19115 ---- Animal proteins ---- CX3C chemokine receptor 1 Source.60: DFBPPR19171 ---- Animal proteins ---- PCI domain-containing protein 2 Source.61: DFBPPR19247 ---- Animal proteins ---- Calmegin Source.62: DFBPPR19365 ---- Animal proteins ---- Inactive rhomboid protein 1 Source.63: DFBPPR19821 ---- Animal proteins ---- Glutamine-dependent NAD(+) synthetase Source.64: DFBPPR20256 ---- Animal proteins ---- Leukocyte surface antigen CD53 Source.65: DFBPPR20528 ---- Animal proteins ---- Thiamin pyrophosphokinase 1 Source.66: DFBPPR20578 ---- Animal proteins ---- Protein rogdi homolog Source.67: DFBPPR20612 ---- Animal proteins ---- Dolichol kinase Source.68: DFBPPR20626 ---- Animal proteins ---- Melanocortin receptor 5 Source.69: DFBPPR20642 ---- Animal proteins ---- Probable ATP-dependent RNA helicase DDX52 Source.70: DFBPPR21154 ---- Animal proteins ---- Proton-activated chloride channel Source.71: DFBPPR21334 ---- Animal proteins ---- LisH domain-containing protein ARMC9 Source.72: DFBPPR21410 ---- Animal proteins ---- Trans-2,3-enoyl-CoA reductase-like Source.73: DFBPPR21418 ---- Animal proteins ---- Angiopoietin-related protein 1 Source.74: DFBPPR21447 ---- Animal proteins ---- Transmembrane protein 39A Source.75: DFBPPR21656 ---- Animal proteins ---- Thioredoxin domain-containing protein 11 Source.76: DFBPPR21725 ---- Animal proteins ---- TBCC domain-containing protein 1 Source.77: DFBPPR22625 ---- Animal proteins ---- Transmembrane protein 164 Source.78: DFBPPR8529 ---- Animal proteins ---- Aspartate aminotransferase, cytoplasmic Source.79: DFBPPR9114 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.80: DFBPPR9196 ---- Animal proteins ---- Steroid 21-hydroxylase Source.81: DFBPPR9209 ---- Animal proteins ---- Cytochrome b-245 light chain Source.82: DFBPPR9242 ---- Animal proteins ---- Carboxypeptidase B Source.83: DFBPPR9753 ---- Animal proteins ---- Syntaxin-binding protein 2 Source.84: DFBPPR9994 ---- Animal proteins ---- Nuclear factor NF-kappa-B p105 subunit Source.85: DFBPPR10116 ---- Animal proteins ---- Cadherin-2 Source.86: DFBPPR10503 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.87: DFBPPR10686 ---- Animal proteins ---- Collectin-11 Source.88: DFBPPR11438 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.89: DFBPPR11576 ---- Animal proteins ---- V-set and immunoglobulin domain-containing protein 1 Source.90: DFBPPR11891 ---- Animal proteins ---- TBCC domain-containing protein 1 Source.91: DFBPPR12384 ---- Animal proteins ---- Calcium-independent phospholipase A2-gamma Source.92: DFBPPR12392 ---- Animal proteins ---- Large neutral amino acids transporter small subunit 2 Source.93: DFBPPR12488 ---- Animal proteins ---- 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase Source.94: DFBPPR12641 ---- Animal proteins ---- Cytochrome b-245 light chain Source.95: DFBPPR12649 ---- Animal proteins ---- C-C chemokine receptor type 5 Source.96: DFBPPR12881 ---- Animal proteins ---- CX3C chemokine receptor 1 Source.97: DFBPPR13202 ---- Animal proteins ---- Aspartate aminotransferase, cytoplasmic Source.98: DFBPPR13259 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.99: DFBPPR13579 ---- Animal proteins ---- Follicle-stimulating hormone receptor Source.100: DFBPPR13892 ---- Animal proteins ---- Melanocortin receptor 5 Source.101: DFBPPR15142 ---- Microorganism protein ---- Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Source.102: DFBPPR15144 ---- Microorganism protein ---- Palmitoyltransferase PFA4 Source.103: DFBPPR15174 ---- Microorganism protein ---- ATP-dependent rRNA helicase RRP3 Source.104: DFBPPR15217 ---- Microorganism protein ---- GPI mannosyltransferase 1 Source.105: DFBPPR15429 ---- Microorganism protein ---- 54S ribosomal protein L2, mitochondrial Source.106: DFBPPR15496 ---- Microorganism protein ---- Cell division cycle protein 123 Source.107: DFBPPR15512 ---- Microorganism protein ---- Vacuolar membrane-associated protein IML1 Source.108: DFBPPR15531 ---- Microorganism protein ---- DNA replication complex GINS protein SLD5 Source.109: DFBPPR15718 ---- Microorganism protein ---- RF4 protein Source.110: DFBPPR15839 ---- Microorganism protein ---- Citrate synthase Source.111: DFBPPR7796 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.112: DFBPPR7821 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic Source.113: DFBPPR7874 ---- Plant protein ---- CASP-like protein 1D1 Source.114: DFBPPR7945 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.115: DFBPPR8086 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.116: DFBPPR8250 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic Source.117: DFBPPR8283 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 42 mM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptides were separated from pepsin digests of soybean proteins by ion exchange chromatography and gel filtration.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang, W., de Mejia, E.G. A New Frontier in Soy Bioactive Peptides that May Prevent Age-related Chronic Diseases. Comprehensive Reviews in Food Science and Food Safety. 2005, 4, 63-78. DOI: 10.1111/j.1541-4337.2005.tb00075.x
Other literature(s)	N.D
PubDate	2005