Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0791(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0791
Peptide sequence	YKYY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Tyr-Lys-Tyr-Tyr

Single-letter amino acid

YKYY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	635.71 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.39 ^c

IC₅₀

64.2 uM

pIC₅₀

-1.808

GRAVY

-1.9500^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine
Organism/Source	Wakame (Undaria pinnatifida)
Precursor protein	Wake hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1077 ---- Plant proteins ---- Hexokinase-9 Source.2: DFBPPR1116 ---- Plant proteins ---- Polycomb group protein EMF2B Source.3: DFBPPR1869 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 8, mitochondrial Source.4: DFBPPR2089 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 3, mitochondrial Source.5: DFBPPR2660 ---- Plant proteins ---- ABC transporter G family member 25 Source.6: DFBPPR6306 ---- Plant proteins ---- NADP-dependent glyceraldehyde-3-phosphate dehydrogenase Source.7: DFBPPR6636 ---- Plant proteins ---- NADP-dependent glyceraldehyde-3-phosphate dehydrogenase Source.8: DFBPPR7642 ---- Milk proteins ---- Inactive pancreatic lipase-related protein 1 Source.9: DFBPPR8508 ---- Milk proteins ---- Pancreatic lipase-related protein 2 Source.10: DFBPPR15994 ---- Animal proteins ---- Inactive pancreatic lipase-related protein 1 Source.11: DFBPPR17195 ---- Animal proteins ---- Receptor for retinol uptake STRA6 Source.12: DFBPPR19328 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 12 Source.13: DFBPPR21804 ---- Animal proteins ---- Myelin and lymphocyte protein Source.14: DFBPPR9020 ---- Animal proteins ---- Pancreatic triacylglycerol lipase Source.15: DFBPPR12923 ---- Animal proteins ---- Pancreatic triacylglycerol lipase Source.16: DFBPPR13246 ---- Animal proteins ---- Pancreatic triacylglycerol lipase Source.17: DFBPPR13994 ---- Animal proteins ---- Receptor-type tyrosine-protein phosphatase C Source.18: DFBPPR14130 ---- Marine protein ---- Ubiquitin carboxyl-terminal hydrolase 12 Source.19: DFBPPR14903 ---- Microorganism protein ---- Transcription elongation factor SPT6 Source.20: DFBPPR15263 ---- Microorganism protein ---- Transcriptional regulator PUL4 Source.21: DFBPPR15540 ---- Microorganism protein ---- Probable intron-encoded endonuclease aI3 Source.22: DFBPPR15706 ---- Microorganism protein ---- Mitochondrial translation factor ATP22
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 64.2 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The peptide was separated from the peptic digest of protein prepared from wakame (Undaria pinnatifida).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Wakame is the most popular edible seaweed in Japan since ancient times, so that its safety is established. The purified peptide YKYY is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

DFBP

[D1]	DFBPANHY0024
[D2]	DFBPMUFU0354

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Suetsuna, K., Nakano, T. Identification of an antihypertensive peptide from peptic digest of wakame (Undaria pinnatifida). The Journal of Nutritional Biochemistry. 2000, 11, 450-4. DOI: 10.1016/s0955-2863(00)00110-8
Other literature(s)	[1] Shimizu M. Nutraceutical proteins and peptides in health and disease[J]. ACE Inhibitory Peptides, 2006:269-315.
PubDate	2000