Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0856(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0856
Peptide sequence	YPQRDMPIQAFLLYQEPV
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Pro-Gln-Arg-Asp-Met-Pro-Ile-Gln-Ala-Phe-Leu-Leu-Tyr-Gln-Glu-Pro-Val

Single-letter amino acid

YPQRDMPIQAFLLYQEPV

Peptide length

Peptide mass

Experimental mass	Theoretical mass
2211.00 Da	2208.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

4.12 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.3667^c

Hydrophilic residue ratio

55.56%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	β-Casein
Residue position	f(180-197)
Precursor protein(s) search	Source.1: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was potential Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory peptide (IC₅₀ value not determined).
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N.D

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Enzyme-modified cheese treated with Neutrase and Debitrase for 96 h.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Haileselassie SS, Lee BH, Gibbs BF. Purification and identification of potentially bioactive peptides from enzyme-modified cheese. J Dairy Sci. 1999 Aug;82(8):1612-7. PMID: 10480087 DOI: 10.3168/jds.S0022-0302(99)75389-0
Other literature(s)	[1] Hayes M, Stanton C, Fitzgerald GF, Ross RP. Putting microbes to work: dairy fermentation, cell factories and bioactive peptides. Part II: bioactive peptide functions. Biotechnol J. 2007 Apr;2(4):435-49.
PubDate	1999