Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0887(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0887
Peptide sequence	KVLILA
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Val-Leu-Ile-Leu-Ala

Single-letter amino acid

KVLILA

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	655.86 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

20.85 uM

pIC₅₀

-1.319

GRAVY

2.3667^c

Hydrophilic residue ratio

83.33%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	Casein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR7605 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7665 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7681 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.5: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.6: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.7: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.8: DFBPPR13067 ---- Animal proteins ---- Beta-casein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 20.85 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptide was synthesized by conventional solid-phase chemistry.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Human testicular ACE (tACE) in complex with captopril (PDB code: 1UZF) was used as a receptor for virtual screening.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang, Z.-l., Zhang, S.-s., Wang, W., Feng, F.-q., Shan, W.-g. A Novel Angiotensin I Converting Enzyme Inhibitory Peptide from the Milk Casein: Virtual Screening and Docking Studies. Agricultural Sciences in China. 2011, 10, 463-7. DOI: 10.1016/S1671-2927(11)60026-6
Other literature(s)	N.D
PubDate	2011