Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0889(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0889
Peptide sequence	VRSP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Val-Arg-Ser-Pro

Single-letter amino acid

VRSP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	457.52 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.04 ^c

IC₅₀

2018.27 uM

pIC₅₀

-3.305

GRAVY

-0.6750^c

Hydrophilic residue ratio

50%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	Casein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1012 ---- Plant proteins ---- Kinesin-like protein KIN-7D, chloroplastic Source.2: DFBPPR1184 ---- Plant proteins ---- Lysine-specific demethylase SE14 Source.3: DFBPPR1306 ---- Plant proteins ---- Two pore calcium channel protein 1 Source.4: DFBPPR1361 ---- Plant proteins ---- Anthranilate synthase beta subunit 2, chloroplastic Source.5: DFBPPR1363 ---- Plant proteins ---- Protein YELLOW LEAF 1, choloroplastic Source.6: DFBPPR1400 ---- Plant proteins ---- Cellulose synthase-like protein D4 Source.7: DFBPPR1485 ---- Plant proteins ---- Pheophorbide a oxygenase, chloroplastic Source.8: DFBPPR1776 ---- Plant proteins ---- Lon protease homolog, mitochondrial Source.9: DFBPPR1781 ---- Plant proteins ---- Stemar-13-ene synthase Source.10: DFBPPR1784 ---- Plant proteins ---- Pentatricopeptide repeat-containing protein OTP51, chloroplastic Source.11: DFBPPR1820 ---- Plant proteins ---- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2B Source.12: DFBPPR2004 ---- Plant proteins ---- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2A Source.13: DFBPPR2349 ---- Plant proteins ---- Coatomer subunit gamma-1 Source.14: DFBPPR2550 ---- Plant proteins ---- Polyamine transporter PUT1 Source.15: DFBPPR2611 ---- Plant proteins ---- Probable protein phosphatase 2C 57 Source.16: DFBPPR2780 ---- Plant proteins ---- Coatomer subunit gamma-2 Source.17: DFBPPR2809 ---- Plant proteins ---- Retinoblastoma-related protein 1 Source.18: DFBPPR2848 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta'' Source.19: DFBPPR3373 ---- Plant proteins ---- 4-coumarate--CoA ligase-like 1 Source.20: DFBPPR3425 ---- Plant proteins ---- Transcription initiation factor TFIID subunit 1 Source.21: DFBPPR3656 ---- Plant proteins ---- Kinesin-like protein KIN-7C Source.22: DFBPPR3805 ---- Plant proteins ---- Putative inactive kinesin-like protein KIN-7B Source.23: DFBPPR4061 ---- Plant proteins ---- Chaperone protein ClpC4, chloroplastic Source.24: DFBPPR4453 ---- Plant proteins ---- Protein EXECUTER 1, chloroplastic Source.25: DFBPPR4689 ---- Plant proteins ---- Probable plastid-lipid-associated protein 2, chloroplastic Source.26: DFBPPR5097 ---- Plant proteins ---- Phenylalanine ammonia-lyase 1 Source.27: DFBPPR5138 ---- Plant proteins ---- Subtilisin-like protease Glyma18g48580 Source.28: DFBPPR5415 ---- Plant proteins ---- Eudesmanediol synthase Source.29: DFBPPR5634 ---- Plant proteins ---- Eudesmanediol synthase Source.30: DFBPPR5647 ---- Plant proteins ---- V-type proton ATPase catalytic subunit A Source.31: DFBPPR5699 ---- Plant proteins ---- Sucrose-phosphate synthase Source.32: DFBPPR5854 ---- Plant proteins ---- Retinoblastoma-related protein 3 Source.33: DFBPPR6082 ---- Plant proteins ---- Zein-alpha 19D1 Source.34: DFBPPR6513 ---- Plant proteins ---- Plastoglobulin-1, chloroplastic Source.35: DFBPPR6741 ---- Plant proteins ---- Fructan 6-exohydrolase Source.36: DFBPPR6839 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta'' Source.37: DFBPPR6867 ---- Plant proteins ---- Retinoblastoma-related protein 1 Source.38: DFBPPR7205 ---- Plant proteins ---- DNA-directed RNA polymerase subunit beta'' Source.39: DFBPPR7639 ---- Milk proteins ---- MICAL-like protein 2 Source.40: DFBPPR7686 ---- Milk proteins ---- Kappa-casein Source.41: DFBPPR7695 ---- Milk proteins ---- Kappa-casein Source.42: DFBPPR7715 ---- Milk proteins ---- Kappa-casein Source.43: DFBPPR8492 ---- Milk proteins ---- Kappa-casein Source.44: DFBPPR15939 ---- Animal proteins ---- Rhodopsin Source.45: DFBPPR16104 ---- Animal proteins ---- Retinal guanylyl cyclase 1 Source.46: DFBPPR16760 ---- Animal proteins ---- Carnitine O-acetyltransferase Source.47: DFBPPR16809 ---- Animal proteins ---- Rhodopsin Source.48: DFBPPR16970 ---- Animal proteins ---- Galactoside alpha-(1,2)-fucosyltransferase 1 Source.49: DFBPPR17033 ---- Animal proteins ---- Monoacylglycerol lipase ABHD2 Source.50: DFBPPR17439 ---- Animal proteins ---- Folliculin Source.51: DFBPPR17853 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL3 Source.52: DFBPPR18110 ---- Animal proteins ---- Protein inturned Source.53: DFBPPR18547 ---- Animal proteins ---- AP-2 complex subunit mu Source.54: DFBPPR18946 ---- Animal proteins ---- Paternally-expressed gene 3 protein Source.55: DFBPPR19669 ---- Animal proteins ---- Cullin-associated NEDD8-dissociated protein 1 Source.56: DFBPPR19730 ---- Animal proteins ---- Tensin-1 Source.57: DFBPPR21270 ---- Animal proteins ---- Mitochondrial import inner membrane translocase subunit Tim21 Source.58: DFBPPR21511 ---- Animal proteins ---- GRB2-associated-binding protein 1 Source.59: DFBPPR21587 ---- Animal proteins ---- Abnormal spindle-like microcephaly-associated protein homolog Source.60: DFBPPR21769 ---- Animal proteins ---- Zinc finger FYVE domain-containing protein 21 Source.61: DFBPPR22085 ---- Animal proteins ---- Zinc finger protein 512 Source.62: DFBPPR22461 ---- Animal proteins ---- Ashwin Source.63: DFBPPR22658 ---- Animal proteins ---- Protein phosphatase 1 regulatory subunit 32 Source.64: DFBPPR22702 ---- Animal proteins ---- Coiled-coil domain-containing protein 83 Source.65: DFBPPR8634 ---- Animal proteins ---- Vitamin K-dependent protein C Source.66: DFBPPR8717 ---- Animal proteins ---- Rhodopsin Source.67: DFBPPR9533 ---- Animal proteins ---- Protamine-2 Source.68: DFBPPR9692 ---- Animal proteins ---- Mitochondria-eating protein Source.69: DFBPPR10138 ---- Animal proteins ---- Epidermal growth factor receptor Source.70: DFBPPR10297 ---- Animal proteins ---- Vitellogenin-2 Source.71: DFBPPR10304 ---- Animal proteins ---- Rhodopsin Source.72: DFBPPR10358 ---- Animal proteins ---- Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Source.73: DFBPPR10418 ---- Animal proteins ---- Green-sensitive opsin Source.74: DFBPPR10538 ---- Animal proteins ---- Retinoblastoma-associated protein Source.75: DFBPPR10555 ---- Animal proteins ---- Fanconi anemia group M protein Source.76: DFBPPR10703 ---- Animal proteins ---- Tensin Source.77: DFBPPR10813 ---- Animal proteins ---- Neurofilament medium polypeptide Source.78: DFBPPR10905 ---- Animal proteins ---- Probable G-protein coupled receptor 149 Source.79: DFBPPR10928 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 28 Source.80: DFBPPR11013 ---- Animal proteins ---- Band 3 anion transport protein Source.81: DFBPPR11026 ---- Animal proteins ---- AP-2 complex subunit mu Source.82: DFBPPR11042 ---- Animal proteins ---- Target of rapamycin complex 2 subunit MAPKAP1 Source.83: DFBPPR11295 ---- Animal proteins ---- Histone H2A deubiquitinase MYSM1 Source.84: DFBPPR11626 ---- Animal proteins ---- tRNA-splicing endonuclease subunit Sen2 Source.85: DFBPPR11752 ---- Animal proteins ---- Actin-related protein 5 Source.86: DFBPPR12098 ---- Animal proteins ---- NEDD4-binding protein 1 Source.87: DFBPPR12165 ---- Animal proteins ---- Vacuolar fusion protein MON1 homolog A Source.88: DFBPPR12417 ---- Animal proteins ---- Rhodopsin Source.89: DFBPPR12430 ---- Animal proteins ---- Junctophilin-2 Source.90: DFBPPR13605 ---- Animal proteins ---- Rhodopsin Source.91: DFBPPR13936 ---- Animal proteins ---- Abnormal spindle-like microcephaly-associated protein homolog Source.92: DFBPPR14370 ---- Marine protein ---- DNA-directed RNA polymerase subunit beta'' Source.93: DFBPPR14855 ---- Marine protein ---- Rhodopsin, freshwater form Source.94: DFBPPR14856 ---- Marine protein ---- Rhodopsin, deep-sea form Source.95: DFBPPR14983 ---- Microorganism protein ---- PAN2-PAN3 deadenylation complex subunit PAN3 Source.96: DFBPPR14994 ---- Microorganism protein ---- Serine/threonine-protein kinase MEC1 Source.97: DFBPPR15723 ---- Microorganism protein ---- Regulator of free ubiquitin chains 1 Source.98: DFBPPR15758 ---- Microorganism protein ---- Required for respiratory growth protein 8, mitochondrial Source.99: DFBPPR7903 ---- Plant protein ---- CASP-like protein 4U1
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide showed almost no Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 2018.27 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])(CO)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptide was synthesized by conventional solid-phase chemistry.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Human testicular ACE (tACE) in complex with captopril (PDB code: 1UZF) was used as a receptor for virtual screening.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Wang, Z.-l., Zhang, S.-s., Wang, W., Feng, F.-q., Shan, W.-g. A Novel Angiotensin I Converting Enzyme Inhibitory Peptide from the Milk Casein: Virtual Screening and Docking Studies. Agricultural Sciences in China. 2011, 10, 463-7. DOI: 10.1016/S1671-2927(11)60026-6
Other literature(s)	N.D
PubDate	2011