Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0943(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0943
Peptide sequence	LIVTQ
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Ile-Val-Thr-Gln

Single-letter amino acid

LIVTQ

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	572.70 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

0.087 uM

pIC₅₀

1.06

GRAVY

1.6600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soy
Precursor protein	Soy protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR2857 ---- Plant proteins ---- ABC transporter G family member 42 Source.2: DFBPPR5547 ---- Plant proteins ---- Methylenetetrahydrofolate reductase 1 Source.3: DFBPPR7013 ---- Plant proteins ---- Protein MLO Source.4: DFBPPR7252 ---- Plant proteins ---- MLO protein homolog 1 Source.5: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 0.087 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(CCC(=O)N)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Soy was fermentated with Lactobacillus casei spp. Pseudoplantarum.

Stability & Cytotoxicity

Peptide stability

Literature report:	The LIVTQ peptide retains ACE inhibitory activity after the action of pepsin. The dialyzability studies by in vitro method show that the rate of absorption/dialyzability was slow.
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The purified peptide LIVTQ is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	S Vallabha, V., Tiku, P.K. Antihypertensive Peptides Derived from Soy Protein by Fermentation. International Journal of Peptide Research and Therapeutics. 2013, 20, 161-8. DOI: 10.1007/s10989-013-9377-5
Other literature(s)	N.D
PubDate	2013