Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0970(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0970
Peptide sequence	AAATP
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], DPP IV-inhibitory activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Ala-Ala-Ala-Thr-Pro

Single-letter amino acid

AAATP

Peptide length

Peptide mass

Experimental mass	Theoretical mass
428.02 Da	429.46 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

100 uM

pIC₅₀

-2.0

GRAVY

0.6200^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Spanish dry-cured ham
Precursor protein	Allantoicase Sus scrofa
Residue position	f(133-137)
Precursor protein(s) search	Source.1: DFBPPR1100 ---- Plant proteins ---- Soluble starch synthase 2-3, chloroplastic/amyloplastic Source.2: DFBPPR1180 ---- Plant proteins ---- Anthranilate synthase beta subunit 1, chloroplastic Source.3: DFBPPR1256 ---- Plant proteins ---- Cytochrome P450 78A11 Source.4: DFBPPR1418 ---- Plant proteins ---- SCARECROW-LIKE protein 7 Source.5: DFBPPR1662 ---- Plant proteins ---- Probable allantoate deiminase Source.6: DFBPPR1731 ---- Plant proteins ---- Indole-3-pyruvate monooxygenase YUCCA4 Source.7: DFBPPR1832 ---- Plant proteins ---- ATP-dependent zinc metalloprotease FTSH 7, chloroplastic Source.8: DFBPPR1835 ---- Plant proteins ---- Laccase-15 Source.9: DFBPPR1899 ---- Plant proteins ---- High-affinity nitrate transporter 2.1 Source.10: DFBPPR1909 ---- Plant proteins ---- High-affinity nitrate transporter 2.2 Source.11: DFBPPR2054 ---- Plant proteins ---- NAC domain-containing protein 10 Source.12: DFBPPR2181 ---- Plant proteins ---- 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic Source.13: DFBPPR2389 ---- Plant proteins ---- Nuclear transcription factor Y subunit B-1 Source.14: DFBPPR2428 ---- Plant proteins ---- Bidirectional sugar transporter SWEET13 Source.15: DFBPPR2438 ---- Plant proteins ---- Arabinogalactan protein 1 Source.16: DFBPPR2487 ---- Plant proteins ---- Two-component response regulator ORR2 Source.17: DFBPPR2523 ---- Plant proteins ---- Protein TIC 62, chloroplastic Source.18: DFBPPR2588 ---- Plant proteins ---- Putative heat stress transcription factor B-4a Source.19: DFBPPR2912 ---- Plant proteins ---- Probable auxin efflux carrier component 3b Source.20: DFBPPR2948 ---- Plant proteins ---- Expansin-A25 Source.21: DFBPPR3324 ---- Plant proteins ---- Dehydration-responsive element-binding protein 2C Source.22: DFBPPR3368 ---- Plant proteins ---- Probable zinc metalloprotease EGY1, chloroplastic Source.23: DFBPPR3631 ---- Plant proteins ---- BTB/POZ domain and ankyrin repeat-containing protein NPR5 Source.24: DFBPPR3770 ---- Plant proteins ---- Putative DEAD-box ATP-dependent RNA helicase 51 Source.25: DFBPPR3878 ---- Plant proteins ---- Growth-regulating factor 10 Source.26: DFBPPR3925 ---- Plant proteins ---- Squamosa promoter-binding-like protein 5 Source.27: DFBPPR3971 ---- Plant proteins ---- WUSCHEL-related homeobox 12 Source.28: DFBPPR4102 ---- Plant proteins ---- Cyclase-like protein 3 Source.29: DFBPPR4428 ---- Plant proteins ---- Acyl transferase 9 Source.30: DFBPPR4833 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 56 Source.31: DFBPPR4878 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 10 Source.32: DFBPPR5427 ---- Plant proteins ---- Transcription factor TEOSINTE BRANCHED 1 Source.33: DFBPPR5687 ---- Plant proteins ---- Protein AMEIOTIC 1 Source.34: DFBPPR6263 ---- Plant proteins ---- Chlorophyll a-b binding protein 3, chloroplastic Source.35: DFBPPR6788 ---- Plant proteins ---- Histone H1 Source.36: DFBPPR6959 ---- Plant proteins ---- Ninja-family protein 2 Source.37: DFBPPR6987 ---- Plant proteins ---- Ninja-family protein 1 Source.38: DFBPPR6991 ---- Plant proteins ---- Ninja-family protein 3 Source.39: DFBPPR7004 ---- Plant proteins ---- Uncharacterized 16 kDa protein in middle repetitive insertion sequence WIS1 Source.40: DFBPPR7254 ---- Plant proteins ---- Plastocyanin, chloroplastic Source.41: DFBPPR16646 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.42: DFBPPR16999 ---- Animal proteins ---- TGF-beta receptor type-1 Source.43: DFBPPR17057 ---- Animal proteins ---- Cation-independent mannose-6-phosphate receptor Source.44: DFBPPR17534 ---- Animal proteins ---- RISC-loading complex subunit TARBP2 Source.45: DFBPPR17741 ---- Animal proteins ---- Polyadenylate-binding protein 1 Source.46: DFBPPR19529 ---- Animal proteins ---- Cbp/p300-interacting transactivator 1 Source.47: DFBPPR20018 ---- Animal proteins ---- MARCKS-related protein Source.48: DFBPPR20506 ---- Animal proteins ---- Akirin-2 Source.49: DFBPPR20685 ---- Animal proteins ---- ER membrane protein complex subunit 10 Source.50: DFBPPR20914 ---- Animal proteins ---- AT-rich interactive domain-containing protein 5B Source.51: DFBPPR21375 ---- Animal proteins ---- Transcription factor jun-D Source.52: DFBPPR21393 ---- Animal proteins ---- mRNA-decapping enzyme 1B Source.53: DFBPPR21400 ---- Animal proteins ---- Replication initiator 1 Source.54: DFBPPR21756 ---- Animal proteins ---- Probable allantoicase Source.55: DFBPPR8776 ---- Animal proteins ---- Serine/threonine-protein kinase WNK1 Source.56: DFBPPR9867 ---- Animal proteins ---- Phostensin Source.57: DFBPPR9995 ---- Animal proteins ---- Melanocyte protein PMEL Source.58: DFBPPR10228 ---- Animal proteins ---- Presenilin-2 Source.59: DFBPPR10536 ---- Animal proteins ---- Inhibin beta B chain Source.60: DFBPPR11970 ---- Animal proteins ---- Rap1 GTPase-activating protein 2 Source.61: DFBPPR12604 ---- Animal proteins ---- MARCKS-related protein
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited high Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 100 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])(C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N1[C@@]([H])(CCC1)C(=O)O

Preparation method

Mode of preparation	Extraction
Enzyme(s)/starter culture	The peptide was obtained from the water-soluble fractions of dry-cured ham.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Spanish dry-cured ham may represent a source of natural peptides able to reduce both ACE inhibitory activity and systolic blood pressure.

Database cross-references

DFBP

[D1]	DFBPANHY0526
[D2]	DFBPDPIV0050
[D3]	DFBPMUFU0210

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Escudero E, Mora L, Fraser PD, Aristoy MC, Arihara K, Toldrá F. Purification and Identification of antihypertensive peptides in Spanish dry-cured ham. J Proteomics. 2013 Jan 14;78:499-507. PMID: 23117181 DOI: 10.1016/j.jprot.2012.10.019
Other literature(s)	N.D
PubDate	2013