Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0976(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0976
Peptide sequence	EGGSF
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Glu-Gly-Gly-Ser-Phe

Single-letter amino acid

EGGSF

Peptide length

Peptide mass

Experimental mass	Theoretical mass
459 Da	495.49 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.4600^c

Hydrophilic residue ratio

60%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Black Bean (Phaseolus vulgaris)
Precursor protein	Precooked Black bean protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1160 ---- Plant proteins ---- Cytochrome P450 90A3 Source.2: DFBPPR1368 ---- Plant proteins ---- Cytochrome P450 90A4 Source.3: DFBPPR8192 ---- Plant proteins ---- 2S albumin Source.4: DFBPPR16524 ---- Animal proteins ---- Suppressor of cytokine signaling 3 Source.5: DFBPPR16900 ---- Animal proteins ---- cAMP-dependent protein kinase type I-alpha regulatory subunit Source.6: DFBPPR18451 ---- Animal proteins ---- Suppressor of cytokine signaling 3 Source.7: DFBPPR18819 ---- Animal proteins ---- Proteasome activator complex subunit 4 Source.8: DFBPPR19291 ---- Animal proteins ---- Multicilin Source.9: DFBPPR20579 ---- Animal proteins ---- Synaptogyrin-3 Source.10: DFBPPR8606 ---- Animal proteins ---- Stimulator of interferon genes protein Source.11: DFBPPR9327 ---- Animal proteins ---- Multicilin Source.12: DFBPPR10098 ---- Animal proteins ---- Mucin-6 Source.13: DFBPPR10450 ---- Animal proteins ---- cAMP-dependent protein kinase type I-alpha regulatory subunit Source.14: DFBPPR11371 ---- Animal proteins ---- Suppressor of cytokine signaling 3 Source.15: DFBPPR12011 ---- Animal proteins ---- Protein Churchill Source.16: DFBPPR14891 ---- Microorganism protein ---- DNA polymerase epsilon catalytic subunit A Source.17: DFBPPR7934 ---- Plant protein ---- Acid beta-fructofuranosidase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was potential Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory peptide (data not shown). The bioactive sequences were SG, GG, EG, SF.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)NCC(=O)NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Precooked Black bean proteins were hydrolyzed with pepsin/pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Mojica L, Chen K, de Mejía EG. Impact of commercial precooking of common bean (Phaseolus vulgaris) on the generation of peptides, after pepsin-pancreatin hydrolysis, capable to inhibit dipeptidyl peptidase-IV. J Food Sci. 2015 Jan;80(1):H188-98. PMID: 25495131 DOI: 10.1111/1750-3841.12726
Other literature(s)	N.D
PubDate	2015