Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI0994(ACE-inhibitory peptide)

DFBP ID	DFBPACEI0994
Peptide sequence	YTGN
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Tyr-Thr-Gly-Asn

Single-letter amino acid

YTGN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
453 Da	453.45 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-1.4750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Great Northern Bean (Phaseolus vulgaris)
Precursor protein	Precooked Great Northern bean protein hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1429 ---- Plant proteins ---- Tubulin beta-4 chain Source.2: DFBPPR1724 ---- Plant proteins ---- Protein disulfide isomerase-like 1-4 Source.3: DFBPPR2136 ---- Plant proteins ---- Alpha-1,3-arabinosyltransferase XAT3 Source.4: DFBPPR2168 ---- Plant proteins ---- DnaJ protein ERDJ2 Source.5: DFBPPR2169 ---- Plant proteins ---- Alpha-1,3-arabinosyltransferase XAT2 Source.6: DFBPPR3138 ---- Plant proteins ---- Villin-1 Source.7: DFBPPR3363 ---- Plant proteins ---- Beta-galactosidase 2 Source.8: DFBPPR3389 ---- Plant proteins ---- Beta-galactosidase 7 Source.9: DFBPPR3440 ---- Plant proteins ---- Agmatine hydroxycinnamoyltransferase 1 Source.10: DFBPPR4535 ---- Plant proteins ---- Protein NLP2 Source.11: DFBPPR4788 ---- Plant proteins ---- Putative B3 domain-containing protein Os03g0621600 Source.12: DFBPPR5776 ---- Plant proteins ---- Tubulin beta-6 chain Source.13: DFBPPR7028 ---- Plant proteins ---- Agmatine coumaroyltransferase-1 Source.14: DFBPPR7240 ---- Plant proteins ---- Agmatine coumaroyltransferase-2 Source.15: DFBPPR16061 ---- Animal proteins ---- Hepatocyte growth factor Source.16: DFBPPR16128 ---- Animal proteins ---- Cubilin Source.17: DFBPPR16498 ---- Animal proteins ---- Aldehyde dehydrogenase, dimeric NADP-preferring Source.18: DFBPPR16527 ---- Animal proteins ---- Contactin-associated protein-like 5 Source.19: DFBPPR16836 ---- Animal proteins ---- Fibronectin Source.20: DFBPPR17025 ---- Animal proteins ---- Tissue-type plasminogen activator Source.21: DFBPPR17515 ---- Animal proteins ---- 5'-nucleotidase Source.22: DFBPPR18024 ---- Animal proteins ---- Kynurenine--oxoglutarate transaminase 3 Source.23: DFBPPR18784 ---- Animal proteins ---- Thrombospondin-4 Source.24: DFBPPR18946 ---- Animal proteins ---- Paternally-expressed gene 3 protein Source.25: DFBPPR18959 ---- Animal proteins ---- Galactose mutarotase Source.26: DFBPPR18988 ---- Animal proteins ---- Lysosomal Pro-X carboxypeptidase Source.27: DFBPPR19175 ---- Animal proteins ---- Corticosteroid-binding globulin Source.28: DFBPPR19416 ---- Animal proteins ---- Gamma-glutamyl hydrolase Source.29: DFBPPR20420 ---- Animal proteins ---- Hepatocyte growth factor Source.30: DFBPPR21413 ---- Animal proteins ---- Protein kinase C-binding protein NELL2 Source.31: DFBPPR22019 ---- Animal proteins ---- ATP-binding cassette sub-family F member 2 Source.32: DFBPPR22121 ---- Animal proteins ---- Transmembrane protein 70, mitochondrial Source.33: DFBPPR22697 ---- Animal proteins ---- MORN repeat-containing protein 2 Source.34: DFBPPR8923 ---- Animal proteins ---- Cubilin Source.35: DFBPPR9335 ---- Animal proteins ---- Galactose mutarotase Source.36: DFBPPR9416 ---- Animal proteins ---- Corticosteroid-binding globulin Source.37: DFBPPR10193 ---- Animal proteins ---- Thy-1 membrane glycoprotein Source.38: DFBPPR10689 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 13 Source.39: DFBPPR10787 ---- Animal proteins ---- Angiogenin Source.40: DFBPPR11402 ---- Animal proteins ---- Contactin-associated protein-like 5 Source.41: DFBPPR11953 ---- Animal proteins ---- Protein NEL Source.42: DFBPPR12384 ---- Animal proteins ---- Calcium-independent phospholipase A2-gamma Source.43: DFBPPR13770 ---- Animal proteins ---- Corticosteroid-binding globulin Source.44: DFBPPR15296 ---- Microorganism protein ---- High-affinity glucose transporter Source.45: DFBPPR15784 ---- Microorganism protein ---- Uncharacterized killer plasmid pGKl-2 protein 1 Source.46: DFBPPR15849 ---- Microorganism protein ---- Exoglucanase Source.47: DFBPPR8075 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit K, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide was potential Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory peptide (data not shown). The bioactive sequence was TG.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Precooked Great Northern bean proteins were hydrolyzed with pepsin/pancreatin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Mojica L, Chen K, de Mejía EG. Impact of commercial precooking of common bean (Phaseolus vulgaris) on the generation of peptides, after pepsin-pancreatin hydrolysis, capable to inhibit dipeptidyl peptidase-IV. J Food Sci. 2015 Jan;80(1):H188-98. PMID: 25495131 DOI: 10.1111/1750-3841.12726
Other literature(s)	N.D
PubDate	2015