Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1031(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1031
Peptide sequence	LSGNK
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antioxidative activity ^[D1], Anticancer activity ^[D2], Multifunctional activity ^[D3]

Calculated physicochemical properties

Three-letter amino acid

Leu-Ser-Gly-Asn-Lys

Single-letter amino acid

LSGNK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	517.58 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.04 ^c

IC₅₀

130.6 ± 1.56 μM

pIC₅₀

-2.116

GRAVY

-0.9600^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Common beans (Phaseolus vulgaris L.)
Precursor protein	Non-digestible fraction of common beans
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR3070 ---- Plant proteins ---- Putative D-cysteine desulfhydrase 1, mitochondrial Source.2: DFBPPR4668 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 62 Source.3: DFBPPR15995 ---- Animal proteins ---- Ras-related protein Rab-5A Source.4: DFBPPR17263 ---- Animal proteins ---- E3 ubiquitin-protein ligase UHRF1 Source.5: DFBPPR17328 ---- Animal proteins ---- Ras-related protein Rab-5A Source.6: DFBPPR17954 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member A Source.7: DFBPPR20098 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member B Source.8: DFBPPR21047 ---- Animal proteins ---- WD repeat-containing protein 48 Source.9: DFBPPR22091 ---- Animal proteins ---- Ankyrin repeat and MYND domain-containing protein 2 Source.10: DFBPPR8722 ---- Animal proteins ---- Ras-related protein Rab-5A Source.11: DFBPPR9415 ---- Animal proteins ---- Iron-responsive element-binding protein 2 Source.12: DFBPPR10930 ---- Animal proteins ---- WD repeat-containing protein 48 Source.13: DFBPPR11423 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member E Source.14: DFBPPR11441 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member B Source.15: DFBPPR12163 ---- Animal proteins ---- Ankyrin repeat and MYND domain-containing protein 2 Source.16: DFBPPR12520 ---- Animal proteins ---- Cytochrome P450 4A4 Source.17: DFBPPR13867 ---- Animal proteins ---- Acidic leucine-rich nuclear phosphoprotein 32 family member B Source.18: DFBPPR15486 ---- Microorganism protein ---- Transcription factor IWS1 Source.19: DFBPPR7930 ---- Plant protein ---- Glucose-1-phosphate adenylyltransferase small subunit 1, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 130.6 ± 1.56 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(CO)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Synthesis
Enzyme(s)/starter culture	The peptide LSGNK was synthesized by GenScript (Piscataway, NJ, USA) with a purity of > 98% based on the composition found in the non-digestible fraction of common beans.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

Docking calculations were carried out on human testicular angiotensin I-converting enzyme (PDB ID: 1UZE) protein models.

Database cross-references

DFBP

[D1]	DFBPANOX0517
[D2]	DFBPANCA0018
[D3]	DFBPMUFU0527

[D4]

[D5]

[D6]

[D7]

Reference(s)

Primary literature	Luna-Vital DA, González de Mejía E, Mendoza S, Loarca-Piña G. Peptides present in the non-digestible fraction of common beans (Phaseolus vulgaris L.) inhibit the angiotensin-I converting enzyme by interacting with its catalytic cavity independent of their antioxidant capacity. Food Funct. 2015 May;6(5):1470-9. PMID: 25881860 DOI: 10.1039/c5fo00190k
Other literature(s)	N.D
PubDate	2015