DFBP ID - DFBPACEI1035(ACE-inhibitory peptide) |
DFBP ID |
DFBPACEI1035 |
Peptide sequence |
RPYL |
Type |
Native peptide |
Peptide/Function name |
ACE-inhibitory peptide |
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Function-activity relationship |
Main bioactivity |
ACE-inhibitory activity |
Otheir bioactivity |
Antihypertensive activity [D1], Multifunctional activity [D2] |
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Calculated physicochemical properties |
Three-letter amino acid |
Arg-Pro-Tyr-Leu |
Single-letter amino acid |
RPYL |
Peptide length |
4 |
Peptide mass |
Experimental mass |
Theoretical mass |
547.2 Da |
547.64 Da c |
|
Net charge |
0.00 c |
Isoelectric point (pI) |
9.82 c |
IC50 |
56.5 ± 1.9 uM |
pIC50 |
-1.752 |
GRAVY |
-0.9000 c |
Hydrophilic residue ratio |
50% c |
Peptide calculator |
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Biological/Functional activity & target protein |
ACE-inhibitory activity |
The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC50
value of 56.5 ± 1.9 μM. |
Specific target protein(s) |
Specific Target Protein(s): Angiotensin-converting enzyme |
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Taste properties & Structure |
Bitterness |
Literature report |
N.D |
Bitter prediction tools |
Bitter taste prediction |
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SMILES |
N[C@@]([H])(CCCNC(=N)N)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CC(C)C)C(=O)O |
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Preparation method |
Mode of preparation |
Enzymatic hydrolysis |
Enzyme(s)/starter culture |
Lactoferrin was hydrolyzed with porcine Pepsin. |
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Stability & Cytotoxicity |
Peptide stability |
Literature report: |
The physiological digestion by the stomach and trypsin and brush edge peptidase was simulated, the peptide RPYL was partially hydrolyzed (about 65% of the initial concentration of the input peptide). |
EHP-Tool: |
Enzymatic Hydrolysis Prediction Tool (EHP-Tool) |
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Peptide cytotoxicity |
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Additional information |
Additional information |
The peptide could be applied as nutraceuticals in the context of health-promoting functional foods for the treatment of hypertension. |
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Database cross-references |
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Reference(s) |
Primary literature |
Ruiz-Giménez, P., Salom, J.B., Marcos, J.F., Vallés, S., Martínez-Maqueda, D., Recio, I., et al. Antihypertensive effect of a bovine lactoferrin pepsin hydrolysate: Identification of novel active peptides. Food Chem. 2012, 131, 266-73.
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Other literature(s) |
N.D |
PubDate |
2012 |
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