Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1110(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1110
Peptide sequence	RINKK
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Arg-Ile-Asn-Lys-Lys

Single-letter amino acid

RINKK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	657.81 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

11.68 ^c

IC₅₀

12.05 ± 0.93 μg/mL

pIC₅₀

-1.081

GRAVY

-2.2600^c

Hydrophilic residue ratio

20%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Probiotic yoghurt
Precursor protein	β-Casein
Residue position	f(25-29)
Precursor protein(s) search	Source.1: DFBPPR8489 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR15196 ---- Microorganism protein ---- ATP-dependent RNA helicase DBP8
Link-research Inductive analysis	Link 1: DFBPACEI1724----Bovine milk protein----β-Casein

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 12.05 ± 0.93 μg/mL.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	No prediction can be made about the peptide bitterness. ^prediction

SMILES

N[C@@]([H])(CCCNC(=N)N)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(CC(=O)N)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Yoghurts were prepared either using a sole yoghurt culture including Lactobacillus delbrueckii ssp. bulgaricus Lb1466 and Streptococcus thermophilus St1342, or L. acidophilus L10, L. casei L26 and Bifidobacterium lactis B94 in addition to yoghurt culture.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The development of yoghurt containing higher concentrations of released bioactive ACE inhibitors and viable probiotics may deliver health benefits to consumers.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Donkor, O.N., Henriksson, A., Singh, T.K., Vasiljevic, T., Shah, N.P. ACE-inhibitory activity of probiotic yoghurt. International Dairy Journal. 2007, 17, 1321-31. DOI: 10.1016/j.idairyj.2007.02.009
Other literature(s)	N.D
PubDate	2007