Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1119(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1119
Peptide sequence	GVLY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Val-Leu-Tyr

Single-letter amino acid

GVLY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	450.53 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.92 ^c

IC₅₀

16 ± 1.5 μM

pIC₅₀

-1.204

GRAVY

1.5750^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Hemp (Cannabis sativa L.)
Precursor protein	Defatted hemp seed meals
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0747 ---- Plant proteins ---- 11S globulin seed storage protein Source.2: DFBPPR0776 ---- Plant proteins ---- 11S globulin Source.3: DFBPPR1032 ---- Plant proteins ---- Disease resistance protein RGA4 Source.4: DFBPPR1072 ---- Plant proteins ---- Cytokinin dehydrogenase 2 Source.5: DFBPPR1211 ---- Plant proteins ---- Magnesium-chelatase subunit ChlD, chloroplastic Source.6: DFBPPR1483 ---- Plant proteins ---- Isoamylase 3, chloroplastic Source.7: DFBPPR1872 ---- Plant proteins ---- Cytokinin dehydrogenase 5 Source.8: DFBPPR1894 ---- Plant proteins ---- Clathrin heavy chain 1 Source.9: DFBPPR1898 ---- Plant proteins ---- Clathrin heavy chain 2 Source.10: DFBPPR2073 ---- Plant proteins ---- ABC transporter G family member 43 Source.11: DFBPPR2162 ---- Plant proteins ---- Very-long-chain aldehyde decarbonylase GL1-7 Source.12: DFBPPR2315 ---- Plant proteins ---- Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY Source.13: DFBPPR2350 ---- Plant proteins ---- Metal tolerance protein 4 Source.14: DFBPPR2386 ---- Plant proteins ---- Probable glucuronosyltransferase Os04g0103100 Source.15: DFBPPR2484 ---- Plant proteins ---- Translation factor GUF1 homolog, mitochondrial Source.16: DFBPPR2600 ---- Plant proteins ---- Autophagy protein 5 Source.17: DFBPPR2799 ---- Plant proteins ---- ABC transporter G family member 49 Source.18: DFBPPR2943 ---- Plant proteins ---- Putative 12-oxophytodienoate reductase 8 Source.19: DFBPPR2978 ---- Plant proteins ---- Disease resistance protein RGA4 Source.20: DFBPPR3095 ---- Plant proteins ---- 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4 Source.21: DFBPPR3160 ---- Plant proteins ---- Endoglucanase 11 Source.22: DFBPPR3329 ---- Plant proteins ---- Putative 12-oxophytodienoate reductase 12 Source.23: DFBPPR3330 ---- Plant proteins ---- Putative 12-oxophytodienoate reductase 10 Source.24: DFBPPR3412 ---- Plant proteins ---- CMP-sialic acid transporter 2 Source.25: DFBPPR3495 ---- Plant proteins ---- Putative 12-oxophytodienoate reductase 13 Source.26: DFBPPR3587 ---- Plant proteins ---- Auxin transport protein BIG Source.27: DFBPPR3772 ---- Plant proteins ---- Putative 12-oxophytodienoate reductase 9 Source.28: DFBPPR3909 ---- Plant proteins ---- LIM domain-containing protein PLIM2b Source.29: DFBPPR4056 ---- Plant proteins ---- Probable protein phosphatase 2C 25 Source.30: DFBPPR4078 ---- Plant proteins ---- ERAD-associated E3 ubiquitin-protein ligase component HRD3 Source.31: DFBPPR4194 ---- Plant proteins ---- Potassium transporter 22 Source.32: DFBPPR4216 ---- Plant proteins ---- Prolamin PPROL 14P Source.33: DFBPPR4257 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL4 Source.34: DFBPPR4323 ---- Plant proteins ---- Microtubule-associated protein 70-2 Source.35: DFBPPR4357 ---- Plant proteins ---- Cyclin-F2-2 Source.36: DFBPPR4720 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 8 Source.37: DFBPPR4726 ---- Plant proteins ---- 40S ribosomal protein S10-2 Source.38: DFBPPR4815 ---- Plant proteins ---- 40S ribosomal protein S10-1 Source.39: DFBPPR5185 ---- Plant proteins ---- Actin-1 Source.40: DFBPPR5353 ---- Plant proteins ---- Small heat shock protein, chloroplastic Source.41: DFBPPR5446 ---- Plant proteins ---- Protein OPAQUE1 Source.42: DFBPPR6328 ---- Plant proteins ---- Magnesium-chelatase subunit ChlD, chloroplastic Source.43: DFBPPR6571 ---- Plant proteins ---- Small heat shock protein, chloroplastic Source.44: DFBPPR6775 ---- Plant proteins ---- NADH-ubiquinone oxidoreductase chain 4 Source.45: DFBPPR7085 ---- Plant proteins ---- Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY Source.46: DFBPPR8186 ---- Plant proteins ---- 11S globulin subunit beta Source.47: DFBPPR16145 ---- Animal proteins ---- Pleckstrin homology domain-containing family A member 8 Source.48: DFBPPR16380 ---- Animal proteins ---- Myosin-8 Source.49: DFBPPR16688 ---- Animal proteins ---- Olfactory receptor-like protein OLF4 Source.50: DFBPPR17071 ---- Animal proteins ---- Insulin-like growth factor 1 receptor Source.51: DFBPPR17444 ---- Animal proteins ---- Adhesion G protein-coupled receptor L1 Source.52: DFBPPR17489 ---- Animal proteins ---- Adhesion G protein-coupled receptor L2 Source.53: DFBPPR17873 ---- Animal proteins ---- Rho family-interacting cell polarization regulator 2 Source.54: DFBPPR17941 ---- Animal proteins ---- Hepatocyte growth factor-regulated tyrosine kinase substrate Source.55: DFBPPR18037 ---- Animal proteins ---- Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase Source.56: DFBPPR18079 ---- Animal proteins ---- DNA polymerase beta Source.57: DFBPPR18322 ---- Animal proteins ---- Stomatin-like protein 2, mitochondrial Source.58: DFBPPR18368 ---- Animal proteins ---- E3 ubiquitin-protein ligase TRIP12 Source.59: DFBPPR18792 ---- Animal proteins ---- Integral membrane protein 2C Source.60: DFBPPR18820 ---- Animal proteins ---- LIM domain kinase 2 Source.61: DFBPPR18852 ---- Animal proteins ---- Pleckstrin homology domain-containing family A member 8 Source.62: DFBPPR19211 ---- Animal proteins ---- Beta-synuclein Source.63: DFBPPR19225 ---- Animal proteins ---- 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase Source.64: DFBPPR19314 ---- Animal proteins ---- Probable phospholipid-transporting ATPase IIB Source.65: DFBPPR19415 ---- Animal proteins ---- Coatomer subunit gamma-2 Source.66: DFBPPR19468 ---- Animal proteins ---- Actin-related protein 2/3 complex subunit 2 Source.67: DFBPPR19567 ---- Animal proteins ---- Peroxisomal sarcosine oxidase Source.68: DFBPPR19658 ---- Animal proteins ---- Sodium-independent sulfate anion transporter Source.69: DFBPPR20246 ---- Animal proteins ---- Epsilon-sarcoglycan Source.70: DFBPPR21002 ---- Animal proteins ---- Olfactomedin-like protein 2B Source.71: DFBPPR8604 ---- Animal proteins ---- Alpha-synuclein Source.72: DFBPPR8770 ---- Animal proteins ---- Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase Source.73: DFBPPR9233 ---- Animal proteins ---- Integral membrane protein 2C Source.74: DFBPPR9398 ---- Animal proteins ---- Myosin-2 Source.75: DFBPPR10180 ---- Animal proteins ---- Teneurin-2 Source.76: DFBPPR10290 ---- Animal proteins ---- Trifunctional purine biosynthetic protein adenosine-3 Source.77: DFBPPR10318 ---- Animal proteins ---- LIM domain kinase 1 Source.78: DFBPPR10419 ---- Animal proteins ---- Rho family-interacting cell polarization regulator 2 Source.79: DFBPPR10526 ---- Animal proteins ---- LIM domain kinase 2 Source.80: DFBPPR10561 ---- Animal proteins ---- Lens fiber major intrinsic protein Source.81: DFBPPR11705 ---- Animal proteins ---- SCO-spondin Source.82: DFBPPR11849 ---- Animal proteins ---- Monocarboxylate transporter 9 Source.83: DFBPPR12212 ---- Animal proteins ---- GTPase-activating Rap/Ran-GAP domain-like protein 3 Source.84: DFBPPR12213 ---- Animal proteins ---- Tumor necrosis factor alpha-induced protein 8-like protein 1 Source.85: DFBPPR12326 ---- Animal proteins ---- Phosphatidylcholine-sterol acyltransferase Source.86: DFBPPR13098 ---- Animal proteins ---- Epithelial membrane protein 1 Source.87: DFBPPR13995 ---- Animal proteins ---- Radial spoke head 1 homolog Source.88: DFBPPR14017 ---- Animal proteins ---- Ependymin Source.89: DFBPPR15064 ---- Microorganism protein ---- Palmitoyltransferase SWF1 Source.90: DFBPPR15499 ---- Microorganism protein ---- Mediator of RNA polymerase II transcription subunit 12 Source.91: DFBPPR7791 ---- Plant protein ---- Translation factor GUF1 homolog, mitochondrial Source.92: DFBPPR8229 ---- Plant protein ---- Delta(8)-fatty-acid desaturase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 16 ± 1.5 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Chemical hydrolysis
Enzyme(s)/starter culture	A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Orio LP, Boschin G, Recca T, Morelli CF, Ragona L, Francescato P, Arnoldi A, Speranza G. New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins. J Agric Food Chem. 2017 Dec 6;65(48):10482-10488. PMID: 29112398 DOI: 10.1021/acs.jafc.7b04522
Other literature(s)	N.D
PubDate	2017