Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1145(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1145
Peptide sequence	GSEN
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Gly-Ser-Glu-Asn

Single-letter amino acid

GSEN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	405.36 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

> 1000 uM

pIC₅₀

> -3.0

GRAVY

-2.0500^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Milk protein
Precursor protein	α_s1-Casein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0824 ---- Plant proteins ---- Homeobox-leucine zipper protein ROC2 Source.2: DFBPPR1083 ---- Plant proteins ---- WRKY transcription factor WRKY24 Source.3: DFBPPR1678 ---- Plant proteins ---- Mitogen-activated protein kinase 7 Source.4: DFBPPR1688 ---- Plant proteins ---- UMP-CMP kinase 3 Source.5: DFBPPR1735 ---- Plant proteins ---- Probable aminodeoxychorismate synthase, chloroplastic Source.6: DFBPPR1822 ---- Plant proteins ---- Probable E3 ubiquitin-protein ligase HIP1 Source.7: DFBPPR1941 ---- Plant proteins ---- UMP-CMP kinase 4 Source.8: DFBPPR1968 ---- Plant proteins ---- Kinesin-like protein KIN-14I Source.9: DFBPPR2129 ---- Plant proteins ---- Kinesin-like protein KIN-5C Source.10: DFBPPR2194 ---- Plant proteins ---- U-box domain-containing protein 4 Source.11: DFBPPR2410 ---- Plant proteins ---- Kinesin-like protein KIN-5B Source.12: DFBPPR2455 ---- Plant proteins ---- Auxin response factor 4 Source.13: DFBPPR2876 ---- Plant proteins ---- Kinesin-like protein KIN-5A Source.14: DFBPPR2938 ---- Plant proteins ---- Kinesin-like protein KIN-10A Source.15: DFBPPR3082 ---- Plant proteins ---- 4-alpha-glucanotransferase DPE2 Source.16: DFBPPR3183 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 32 Source.17: DFBPPR3369 ---- Plant proteins ---- Probable adenylate kinase 2, chloroplastic Source.18: DFBPPR3609 ---- Plant proteins ---- Thioredoxin-like 1-1, chloroplastic Source.19: DFBPPR3667 ---- Plant proteins ---- Probable NAD kinase 2, chloroplastic Source.20: DFBPPR3697 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 39 Source.21: DFBPPR4075 ---- Plant proteins ---- Villin-4 Source.22: DFBPPR4639 ---- Plant proteins ---- CASP-like protein 4D1 Source.23: DFBPPR4668 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 62 Source.24: DFBPPR4801 ---- Plant proteins ---- Putative B3 domain-containing protein Os03g0619850 Source.25: DFBPPR4865 ---- Plant proteins ---- P-loop NTPase domain-containing protein LPA1 homolog Source.26: DFBPPR5450 ---- Plant proteins ---- Adenylate kinase, chloroplastic Source.27: DFBPPR7006 ---- Plant proteins ---- WRKY transcription factor SUSIBA2 Source.28: DFBPPR7490 ---- Plant proteins ---- Cysteine proteinase COT44 Source.29: DFBPPR7688 ---- Milk proteins ---- Alpha-S1-casein Source.30: DFBPPR7694 ---- Milk proteins ---- Alpha-S1-casein Source.31: DFBPPR7717 ---- Milk proteins ---- Alpha-S1-casein Source.32: DFBPPR8488 ---- Milk proteins ---- Alpha-S1-casein Source.33: DFBPPR17479 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 13 Source.34: DFBPPR18134 ---- Animal proteins ---- Cyclin-T1 Source.35: DFBPPR18327 ---- Animal proteins ---- Eukaryotic translation initiation factor 6 Source.36: DFBPPR19330 ---- Animal proteins ---- Nuclear pore complex protein Nup85 Source.37: DFBPPR19607 ---- Animal proteins ---- Obg-like ATPase 1 Source.38: DFBPPR19661 ---- Animal proteins ---- Golgi pH regulator Source.39: DFBPPR19825 ---- Animal proteins ---- DNA excision repair protein ERCC-6-like 2 Source.40: DFBPPR19891 ---- Animal proteins ---- Geranylgeranyl transferase type-1 subunit beta Source.41: DFBPPR20467 ---- Animal proteins ---- Tetranectin Source.42: DFBPPR20513 ---- Animal proteins ---- Rho GTPase-activating protein 29 Source.43: DFBPPR20601 ---- Animal proteins ---- Glucocorticoid modulatory element-binding protein 1 Source.44: DFBPPR20643 ---- Animal proteins ---- Fibrinogen-like protein 1 Source.45: DFBPPR20662 ---- Animal proteins ---- C4b-binding protein alpha chain Source.46: DFBPPR20684 ---- Animal proteins ---- Doublesex- and mab-3-related transcription factor A2 Source.47: DFBPPR21004 ---- Animal proteins ---- Cleft lip and palate transmembrane protein 1-like protein Source.48: DFBPPR21265 ---- Animal proteins ---- A-kinase anchor protein 3 Source.49: DFBPPR21953 ---- Animal proteins ---- Clusterin-like protein 1 Source.50: DFBPPR22027 ---- Animal proteins ---- F-box/LRR-repeat protein 4 Source.51: DFBPPR22100 ---- Animal proteins ---- Centromere protein M Source.52: DFBPPR22266 ---- Animal proteins ---- Vexin Source.53: DFBPPR22676 ---- Animal proteins ---- Glycosyltransferase-like domain-containing protein 1 Source.54: DFBPPR8709 ---- Animal proteins ---- Glucocorticoid receptor Source.55: DFBPPR8986 ---- Animal proteins ---- LIM domain and actin-binding protein 1 Source.56: DFBPPR9820 ---- Animal proteins ---- WD repeat-containing protein 62 Source.57: DFBPPR10224 ---- Animal proteins ---- Prolyl 3-hydroxylase 1 Source.58: DFBPPR10354 ---- Animal proteins ---- DNA topoisomerase 2-beta Source.59: DFBPPR10459 ---- Animal proteins ---- Reelin Source.60: DFBPPR10689 ---- Animal proteins ---- Ubiquitin carboxyl-terminal hydrolase 13 Source.61: DFBPPR10888 ---- Animal proteins ---- Nuclear distribution protein nudE-like 1 Source.62: DFBPPR11041 ---- Animal proteins ---- Chromodomain-helicase-DNA-binding protein 7 Source.63: DFBPPR11055 ---- Animal proteins ---- Thymidine kinase, cytosolic Source.64: DFBPPR11204 ---- Animal proteins ---- Protein Hook homolog 1 Source.65: DFBPPR11440 ---- Animal proteins ---- Golgi pH regulator Source.66: DFBPPR11595 ---- Animal proteins ---- Histone-lysine N-methyltransferase SETD1B Source.67: DFBPPR11643 ---- Animal proteins ---- GDNF family receptor alpha-4 Source.68: DFBPPR12085 ---- Animal proteins ---- Lariat debranching enzyme Source.69: DFBPPR12122 ---- Animal proteins ---- Fibroblast growth factor-binding protein 2 Source.70: DFBPPR12309 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase A Source.71: DFBPPR12325 ---- Animal proteins ---- Glucocorticoid receptor Source.72: DFBPPR12382 ---- Animal proteins ---- Solute carrier family 15 member 1 Source.73: DFBPPR12739 ---- Animal proteins ---- Peptidyl-prolyl cis-trans isomerase FKBP3 Source.74: DFBPPR13298 ---- Animal proteins ---- Cyclin-T1 Source.75: DFBPPR13600 ---- Animal proteins ---- Glucocorticoid receptor Source.76: DFBPPR13754 ---- Animal proteins ---- Cytochrome P450 11B1, mitochondrial Source.77: DFBPPR13882 ---- Animal proteins ---- Interferon-induced GTP-binding protein Mx2 Source.78: DFBPPR14171 ---- Marine protein ---- Golgi pH regulator Source.79: DFBPPR15037 ---- Microorganism protein ---- Regulatory protein SWI6 Source.80: DFBPPR15110 ---- Microorganism protein ---- Sterol 3-beta-glucosyltransferase Source.81: DFBPPR15514 ---- Microorganism protein ---- Nucleotide exchange factor SIL1 Source.82: DFBPPR15652 ---- Microorganism protein ---- Translation machinery-associated protein 22
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very weak Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of > 1000 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

NCC(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with an extracellular proteinase from Lactobacillus helveticus CP790.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Yamamoto, N., Akino, A., Takano, T. Antihypertensive Effect of the Peptides Derived from Casein by an Extracellular Proteinase from Lactobacillus helveticus CP790. Journal of Dairy Science. 1994, 77, 917-22. DOI: 10.3168/jds.S0022-0302(94)77026-0
Other literature(s)	N.D
PubDate	1994