Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1158(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1158
Peptide sequence	EMPFPK
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Opioid activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Glu-Met-Pro-Phe-Pro-Lys

Single-letter amino acid

EMPFPK

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	747.90 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

6.92 ^c

IC₅₀

N.D

pIC₅₀

N.D

GRAVY

-0.9833^c

Hydrophilic residue ratio

66.67%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal
Organism/Source	Bovine whey protein
Precursor protein	γ-Casein
Residue position	f(108-113)
Precursor protein(s) search	Source.1: DFBPPR7692 ---- Milk proteins ---- Beta-casein Source.2: DFBPPR7700 ---- Milk proteins ---- Beta-casein Source.3: DFBPPR7718 ---- Milk proteins ---- Beta-casein Source.4: DFBPPR8489 ---- Milk proteins ---- Beta-casein
Link-research Inductive analysis	Link 1: DFBPACEI0558----Bovine milk protein----β-Casein

Biological/Functional activity & target protein

ACE-inhibitory activity

EMPFPK showed an in vitro competitive inhibitor effect on angiotensin-converting enzyme, as shown in table 1.

Table1 Ki values obtained for EMPFPK using E.C. 3.24.15 and ACE enzymes.
Peptide	ACE, Ki (uM)	E.C. 2.24.15, Ki (uM)	References
EMPFPK	14.28	97.2	[0]

Specific target protein(s)

Specific Target Protein(s):
Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCC(=O)O)C(=O)N[C@@]([H])(CCSC)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCCCN)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with trypsin.

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

N.D

Database cross-references

DFBP

[D1]	DFBPOPIO0013
[D2]	DFBPMUFU0135

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Perpetuo, E.A., Juliano, L., Lebrun, I. Biochemical and pharmacological aspects of two bradykinin-potentiating peptides obtained from tryptic hydrolysis of casein. J Protein Chem. 2003, 22, 601-6. DOI: 10.1023/B:JOPC.0000008724.98339.ff
Other literature(s)	N.D
PubDate	2003