Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1180(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1180
Peptide sequence	ALAPE
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	Antihypertensive activity ^[D1], Multifunctional activity ^[D2]

Calculated physicochemical properties

Three-letter amino acid

Ala-Leu-Ala-Pro-Glu

Single-letter amino acid

ALAPE

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	499.55 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.34 ^c

IC₅₀

167.5 uM

pIC₅₀

-2.224

GRAVY

0.4600^c

Hydrophilic residue ratio

80%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Animal, Marine
Organism/Source	Pearl oyster (Pinctada fucata martensii)
Precursor protein	Pearl oyster powder hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1255 ---- Plant proteins ---- Ethylene receptor 3 Source.2: DFBPPR1582 ---- Plant proteins ---- Homeobox-leucine zipper protein HOX4 Source.3: DFBPPR5483 ---- Plant proteins ---- Caffeic acid 3-O-methyltransferase Source.4: DFBPPR18521 ---- Animal proteins ---- Prolyl 4-hydroxylase subunit alpha-3 Source.5: DFBPPR21772 ---- Animal proteins ---- RNA polymerase II-associated protein 1 Source.6: DFBPPR9252 ---- Animal proteins ---- Selenide, water dikinase 2 Source.7: DFBPPR10524 ---- Animal proteins ---- Protein disulfide-isomerase Source.8: DFBPPR12174 ---- Animal proteins ---- Protein CNPPD1 Source.9: DFBPPR14093 ---- Marine protein ---- Hepatocyte nuclear factor 1-alpha
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 167.5 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(C)C(=O)N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])(C)C(=O)N1[C@@]([H])(CCC1)C(=O)N[C@@]([H])(CCC(=O)O)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Hydrolysis with pepsin (Porcine tomach mucosa; Wako Pure Chemicals, Osaka, Japan).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The purified peptide ALAPE is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

DFBP

[D1]	DFBPANHY0845
[D2]	DFBPMUFU0237

[D3]

[D4]

[D5]

[D6]

Reference(s)

Primary literature	Suetsuna, K. Identification of antihypertensive peptides from peptic digest of the short-necked clam Tapes philippinarum and the pearl oyster Pinctada fucata martensii. Fisheries Science. 2002, 68, 233-5. DOI: 10.1046/j.1444-2906.2002.00415.x
Other literature(s)	N.D
PubDate	2002