Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1266(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1266
Peptide sequence	LIVT
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Leu-Ile-Val-Thr

Single-letter amino acid

LIVT

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	444.56 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

5.97 ^c

IC₅₀

0.110 uM

pIC₅₀

0.959

GRAVY

2.9500^c

Hydrophilic residue ratio

75%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Soy
Precursor protein	Soy protein
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0869 ---- Plant proteins ---- Sucrose transport protein SUT1 Source.2: DFBPPR0988 ---- Plant proteins ---- Sucrose synthase 2 Source.3: DFBPPR1209 ---- Plant proteins ---- Aquaporin NIP2-1 Source.4: DFBPPR1557 ---- Plant proteins ---- WRKY transcription factor WRKY51 Source.5: DFBPPR1571 ---- Plant proteins ---- Sucrose transport protein SUT2 Source.6: DFBPPR1611 ---- Plant proteins ---- Fructokinase-2 Source.7: DFBPPR1892 ---- Plant proteins ---- Sucrose synthase 1 Source.8: DFBPPR1999 ---- Plant proteins ---- Signal peptide peptidase-like 4 Source.9: DFBPPR2087 ---- Plant proteins ---- Cytokinin dehydrogenase 10 Source.10: DFBPPR2173 ---- Plant proteins ---- Cullin-1 Source.11: DFBPPR2202 ---- Plant proteins ---- Putative leucine aminopeptidase 1 Source.12: DFBPPR2313 ---- Plant proteins ---- Kinesin-like protein KIN-UA Source.13: DFBPPR2326 ---- Plant proteins ---- Sucrose transport protein SUT3 Source.14: DFBPPR2499 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 5 Source.15: DFBPPR2534 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.16: DFBPPR2582 ---- Plant proteins ---- Probable staphylococcal-like nuclease CAN1 Source.17: DFBPPR2610 ---- Plant proteins ---- Aquaporin NIP2-2 Source.18: DFBPPR2647 ---- Plant proteins ---- Silicon efflux transporter LSI2 Source.19: DFBPPR2672 ---- Plant proteins ---- 63 kDa globulin-like protein Source.20: DFBPPR2757 ---- Plant proteins ---- Probable glucuronosyltransferase Os01g0157700 Source.21: DFBPPR2857 ---- Plant proteins ---- ABC transporter G family member 42 Source.22: DFBPPR2911 ---- Plant proteins ---- Probable V-type proton ATPase subunit d Source.23: DFBPPR3000 ---- Plant proteins ---- Kinesin-like protein KIN-14L Source.24: DFBPPR3395 ---- Plant proteins ---- Probable indole-3-acetic acid-amido synthetase GH3.7 Source.25: DFBPPR3541 ---- Plant proteins ---- Sucrose synthase 3 Source.26: DFBPPR3568 ---- Plant proteins ---- Bidirectional sugar transporter SWEET16 Source.27: DFBPPR3668 ---- Plant proteins ---- DEAD-box ATP-dependent RNA helicase 29 Source.28: DFBPPR3935 ---- Plant proteins ---- Sucrose synthase 4 Source.29: DFBPPR4136 ---- Plant proteins ---- Photosystem II reaction center protein Z Source.30: DFBPPR4257 ---- Plant proteins ---- Probable metal-nicotianamine transporter YSL4 Source.31: DFBPPR4411 ---- Plant proteins ---- Ammonium transporter 2 member 2 Source.32: DFBPPR4637 ---- Plant proteins ---- Putative NAD kinase 3 Source.33: DFBPPR4638 ---- Plant proteins ---- Probable NAD kinase 1 Source.34: DFBPPR5162 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.35: DFBPPR5446 ---- Plant proteins ---- Protein OPAQUE1 Source.36: DFBPPR5547 ---- Plant proteins ---- Methylenetetrahydrofolate reductase 1 Source.37: DFBPPR5842 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.38: DFBPPR5852 ---- Plant proteins ---- Sucrose synthase 2 Source.39: DFBPPR5857 ---- Plant proteins ---- Sucrose synthase 1 Source.40: DFBPPR5870 ---- Plant proteins ---- Protein WRKY1 Source.41: DFBPPR5934 ---- Plant proteins ---- Aquaporin NIP2-1 Source.42: DFBPPR5995 ---- Plant proteins ---- Aquaporin NIP2-2 Source.43: DFBPPR6020 ---- Plant proteins ---- Aquaporin NIP2-3 Source.44: DFBPPR6506 ---- Plant proteins ---- Sucrose synthase 2 Source.45: DFBPPR6527 ---- Plant proteins ---- Photosystem II reaction center protein Z Source.46: DFBPPR6833 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.47: DFBPPR7013 ---- Plant proteins ---- Protein MLO Source.48: DFBPPR7039 ---- Plant proteins ---- Nitrate reductase [NAD(P)H] Source.49: DFBPPR7101 ---- Plant proteins ---- Sucrose synthase 1 Source.50: DFBPPR7106 ---- Plant proteins ---- Sucrose synthase 2 Source.51: DFBPPR7179 ---- Plant proteins ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.52: DFBPPR7252 ---- Plant proteins ---- MLO protein homolog 1 Source.53: DFBPPR8206 ---- Plant proteins ---- DELLA protein GAIP-B Source.54: DFBPPR8499 ---- Milk proteins ---- Beta-lactoglobulin Source.55: DFBPPR8502 ---- Milk proteins ---- Broad substrate specificity ATP-binding cassette transporter ABCG2 Source.56: DFBPPR16529 ---- Animal proteins ---- Carboxylesterase 5A Source.57: DFBPPR16629 ---- Animal proteins ---- Aminomethyltransferase, mitochondrial Source.58: DFBPPR16911 ---- Animal proteins ---- Dihydroorotate dehydrogenase (quinone), mitochondrial Source.59: DFBPPR17024 ---- Animal proteins ---- Sodium-dependent serotonin transporter Source.60: DFBPPR17281 ---- Animal proteins ---- Proteinase-activated receptor 2 Source.61: DFBPPR17354 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.62: DFBPPR17421 ---- Animal proteins ---- Serine protease HTRA2, mitochondrial Source.63: DFBPPR18002 ---- Animal proteins ---- Toll-like receptor 10 Source.64: DFBPPR18027 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.65: DFBPPR18162 ---- Animal proteins ---- Renin receptor Source.66: DFBPPR18641 ---- Animal proteins ---- Cadherin-5 Source.67: DFBPPR18768 ---- Animal proteins ---- Aminomethyltransferase, mitochondrial Source.68: DFBPPR18872 ---- Animal proteins ---- Dipeptidyl aminopeptidase-like protein 6 Source.69: DFBPPR19097 ---- Animal proteins ---- Serine protease HTRA1 Source.70: DFBPPR19187 ---- Animal proteins ---- Activating signal cointegrator 1 complex subunit 3 Source.71: DFBPPR19532 ---- Animal proteins ---- Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 Source.72: DFBPPR19580 ---- Animal proteins ---- Endosomal/lysosomal potassium channel TMEM175 Source.73: DFBPPR19829 ---- Animal proteins ---- Arginine--tRNA ligase, cytoplasmic Source.74: DFBPPR20937 ---- Animal proteins ---- Leucine-rich repeat-containing protein 25 Source.75: DFBPPR20938 ---- Animal proteins ---- Serine protease HTR4 Source.76: DFBPPR21917 ---- Animal proteins ---- Glycosyltransferase 8 domain-containing protein 2 Source.77: DFBPPR22102 ---- Animal proteins ---- Solute carrier family 46 member 3 Source.78: DFBPPR22318 ---- Animal proteins ---- Transmembrane protein 218 Source.79: DFBPPR22326 ---- Animal proteins ---- WD repeat-containing protein 70 Source.80: DFBPPR22372 ---- Animal proteins ---- WD repeat-containing protein 92 Source.81: DFBPPR22526 ---- Animal proteins ---- Testis-expressed protein 29 Source.82: DFBPPR22709 ---- Animal proteins ---- PIH1 domain-containing protein 2 Source.83: DFBPPR8603 ---- Animal proteins ---- Signal transducer and activator of transcription 1 Source.84: DFBPPR8682 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.85: DFBPPR8774 ---- Animal proteins ---- Tenascin Source.86: DFBPPR9634 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.87: DFBPPR9928 ---- Animal proteins ---- Galectin-2 Source.88: DFBPPR9977 ---- Animal proteins ---- Signal transducer and activator of transcription 3 Source.89: DFBPPR10050 ---- Animal proteins ---- Protocadherin-15 Source.90: DFBPPR10271 ---- Animal proteins ---- Cadherin-7 Source.91: DFBPPR10804 ---- Animal proteins ---- Integrin alpha-V Source.92: DFBPPR10872 ---- Animal proteins ---- Inactive tyrosine-protein kinase 7 Source.93: DFBPPR10982 ---- Animal proteins ---- Melatonin receptor type 1C Source.94: DFBPPR11033 ---- Animal proteins ---- Aminomethyltransferase, mitochondrial Source.95: DFBPPR11089 ---- Animal proteins ---- Endosomal/lysosomal potassium channel TMEM175 Source.96: DFBPPR11184 ---- Animal proteins ---- Small RNA 2'-O-methyltransferase Source.97: DFBPPR11204 ---- Animal proteins ---- Protein Hook homolog 1 Source.98: DFBPPR11291 ---- Animal proteins ---- Activating signal cointegrator 1 complex subunit 3 Source.99: DFBPPR11617 ---- Animal proteins ---- DNA repair and recombination protein RAD54B Source.100: DFBPPR11626 ---- Animal proteins ---- tRNA-splicing endonuclease subunit Sen2 Source.101: DFBPPR11634 ---- Animal proteins ---- Melanocyte-stimulating hormone receptor Source.102: DFBPPR11678 ---- Animal proteins ---- Protein ABHD13 Source.103: DFBPPR11825 ---- Animal proteins ---- Solute carrier family 35 member B1 Source.104: DFBPPR12396 ---- Animal proteins ---- Voltage-dependent P/Q-type calcium channel subunit alpha-1A Source.105: DFBPPR12428 ---- Animal proteins ---- Sodium channel protein type 9 subunit alpha Source.106: DFBPPR12565 ---- Animal proteins ---- Protein-glutamine gamma-glutamyltransferase K Source.107: DFBPPR12589 ---- Animal proteins ---- H(+)/Cl(-) exchange transporter 5 Source.108: DFBPPR12802 ---- Animal proteins ---- Complement C3 alpha chain Source.109: DFBPPR14091 ---- Marine protein ---- 40S ribosomal protein SA Source.110: DFBPPR15176 ---- Microorganism protein ---- Cytosolic Fe-S cluster assembly factor NBP35 Source.111: DFBPPR15293 ---- Microorganism protein ---- GPI ethanolamine phosphate transferase 2 Source.112: DFBPPR15588 ---- Microorganism protein ---- Protein PNS1 Source.113: DFBPPR15677 ---- Microorganism protein ---- Ribosome-recycling factor, mitochondrial Source.114: DFBPPR7817 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.115: DFBPPR8009 ---- Plant protein ---- CASP-like protein 5B1 Source.116: DFBPPR8090 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic Source.117: DFBPPR8260 ---- Plant protein ---- NAD(P)H-quinone oxidoreductase subunit H, chloroplastic
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 0.110 uM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CC(C)C)C(=O)N[C@@]([H])([C@]([H])(CC)C)C(=O)N[C@@]([H])(C(C)C)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)O

Preparation method

Mode of preparation	Fermentation
Enzyme(s)/starter culture	Soy was fermentated with Lactobacillus casei spp. Pseudoplantarum.

Stability & Cytotoxicity

Peptide stability

Literature report:	The soy hydrolysates before in vitro digestibility (IC₅₀ value of 0.065 mg/mL), after pepsin action (IC₅₀ value of 0.066 mg/mL) and after pepsin and pancreatic action (IC₅₀ value of 0.073 mg/mL) suggest that the peptide LIVT may be resistant to digestion in gastro intestinal tract.
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The purified peptide LIVT is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	S Vallabha, V., Tiku, P.K. Antihypertensive Peptides Derived from Soy Protein by Fermentation. International Journal of Peptide Research and Therapeutics. 2013, 20, 161-8. DOI: 10.1007/s10989-013-9377-5
Other literature(s)	N.D
PubDate	2013