Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1323(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1323
Peptide sequence	SKTY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Ser-Lys-Thr-Tyr

Single-letter amino acid

SKTY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
498.4 Da	497.54 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

9.70 ^c

IC₅₀

20.63 uM

pIC₅₀

-1.314

GRAVY

-1.6750^c

Hydrophilic residue ratio

0%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Plant
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR0748 ---- Plant proteins ---- Agglutinin Source.2: DFBPPR1279 ---- Plant proteins ---- Homeobox protein HAZ1 Source.3: DFBPPR1565 ---- Plant proteins ---- Nuclear cap-binding protein subunit 1 Source.4: DFBPPR1751 ---- Plant proteins ---- Heat stress transcription factor A-4b Source.5: DFBPPR1919 ---- Plant proteins ---- Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial Source.6: DFBPPR2093 ---- Plant proteins ---- Ent-kaurene oxidase-like 5 Source.7: DFBPPR2310 ---- Plant proteins ---- Heat stress transcription factor A-3 Source.8: DFBPPR2472 ---- Plant proteins ---- Heat stress transcription factor B-4d Source.9: DFBPPR2847 ---- Plant proteins ---- Glutaredoxin-C4, chloroplastic Source.10: DFBPPR3108 ---- Plant proteins ---- Thioredoxin H4-2 Source.11: DFBPPR3186 ---- Plant proteins ---- Endoglucanase 10 Source.12: DFBPPR3471 ---- Plant proteins ---- Monothiol glutaredoxin-S6 Source.13: DFBPPR3775 ---- Plant proteins ---- Kinesin-like protein KIN-14G Source.14: DFBPPR3996 ---- Plant proteins ---- Kinesin-like protein KIN-14J Source.15: DFBPPR5020 ---- Plant proteins ---- Basic 7S globulin Source.16: DFBPPR5046 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 2 Source.17: DFBPPR5049 ---- Plant proteins ---- Inducible nitrate reductase [NADH] 1 Source.18: DFBPPR5140 ---- Plant proteins ---- Basic 7S globulin 2 Source.19: DFBPPR5836 ---- Plant proteins ---- Eukaryotic translation initiation factor 5A Source.20: DFBPPR6469 ---- Plant proteins ---- Oxygen-evolving enhancer protein 1, chloroplastic Source.21: DFBPPR6889 ---- Plant proteins ---- Oxygen-evolving enhancer protein 1, chloroplastic Source.22: DFBPPR7465 ---- Plant proteins ---- Oleosin S1-2 Source.23: DFBPPR15974 ---- Animal proteins ---- Androgen receptor Source.24: DFBPPR15985 ---- Animal proteins ---- von Willebrand factor Source.25: DFBPPR16242 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.26: DFBPPR16320 ---- Animal proteins ---- Guanylate cyclase soluble subunit beta-1 Source.27: DFBPPR16942 ---- Animal proteins ---- Alkaline phosphatase, tissue-nonspecific isozyme Source.28: DFBPPR17121 ---- Animal proteins ---- 3-hydroxyacyl-CoA dehydrogenase type-2 Source.29: DFBPPR17188 ---- Animal proteins ---- Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Source.30: DFBPPR17439 ---- Animal proteins ---- Folliculin Source.31: DFBPPR17745 ---- Animal proteins ---- N-lysine methyltransferase KMT5A Source.32: DFBPPR17804 ---- Animal proteins ---- Calpain-1 catalytic subunit Source.33: DFBPPR17891 ---- Animal proteins ---- Intestinal-type alkaline phosphatase Source.34: DFBPPR18096 ---- Animal proteins ---- Serine palmitoyltransferase 1 Source.35: DFBPPR19040 ---- Animal proteins ---- 26S proteasome non-ATPase regulatory subunit 11 Source.36: DFBPPR19068 ---- Animal proteins ---- CXXC-type zinc finger protein 1 Source.37: DFBPPR19956 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.38: DFBPPR20434 ---- Animal proteins ---- SPRY domain-containing SOCS box protein 1 Source.39: DFBPPR21186 ---- Animal proteins ---- 40S ribosomal protein S2 Source.40: DFBPPR21545 ---- Animal proteins ---- Musculoskeletal embryonic nuclear protein 1 Source.41: DFBPPR22666 ---- Animal proteins ---- Uncharacterized protein C12orf50 homolog Source.42: DFBPPR8867 ---- Animal proteins ---- Calpain-1 catalytic subunit Source.43: DFBPPR9344 ---- Animal proteins ---- Desmoglein-1 Source.44: DFBPPR9555 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.45: DFBPPR10104 ---- Animal proteins ---- Bloom syndrome protein homolog Source.46: DFBPPR10142 ---- Animal proteins ---- Calpain-3 Source.47: DFBPPR10363 ---- Animal proteins ---- E3 ubiquitin-protein ligase HACE1 Source.48: DFBPPR10608 ---- Animal proteins ---- Semaphorin-3D Source.49: DFBPPR10636 ---- Animal proteins ---- LARGE xylosyl- and glucuronyltransferase 1 Source.50: DFBPPR11774 ---- Animal proteins ---- Musculoskeletal embryonic nuclear protein 1 Source.51: DFBPPR11933 ---- Animal proteins ---- M-protein, striated muscle Source.52: DFBPPR12004 ---- Animal proteins ---- Fibronectin type-III domain-containing protein 3a Source.53: DFBPPR12071 ---- Animal proteins ---- Cysteine and histidine-rich domain-containing protein 1 Source.54: DFBPPR12475 ---- Animal proteins ---- Potassium-transporting ATPase subunit beta Source.55: DFBPPR14555 ---- Marine protein ---- Vitellogenin Source.56: DFBPPR15377 ---- Microorganism protein ---- Mitochondrial presequence protease Source.57: DFBPPR15570 ---- Microorganism protein ---- Glucose starvation modulator protein 1 Source.58: DFBPPR8239 ---- Plant protein ---- Serine--tRNA ligase Source.59: DFBPPR8279 ---- Plant protein ---- Oxygen-evolving enhancer protein 1, chloroplastic
Link-research Inductive analysis	Link 1: DFBPACEI2219----Laminaria japonica----Laminaria japonica proteins (LJPs)

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 20.63 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CO)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])([C@]([H])(O)C)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501. DOI: 10.1111/jfpp.12628
Other literature(s)	N.D
PubDate	2016