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List of peptide properties
DFBP ID - DFBPACEI1325(ACE-inhibitory peptide)
DFBP ID DFBPACEI1325
Peptide sequence AKYSY
Type Native peptide
Peptide/Function name ACE-inhibitory peptide
Function-activity relationship
Main bioactivity ACE-inhibitory activity
Otheir bioactivity Antihypertensive activity [D1], Multifunctional activity [D2]
Calculated physicochemical properties
Three-letter amino acid Ala-Lys-Tyr-Ser-Tyr
Single-letter amino acid AKYSY
Peptide length 5
Peptide mass
Experimental mass Theoretical mass
631.5 Da 630.69 Da c
Net charge 0.00 c
Isoelectric point (pI) 9.52 c
IC50

2.42 uM

pIC50 -0.384
GRAVY -1.1000 c
Hydrophilic residue ratio 20% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Marine, Plant
Organism/Source Laminaria japonica
Precursor protein Laminaria japonica hydrolysates
Residue position N.D
Precursor protein(s) search
Link-research
Link 1: DFBPACEI0786----Red algae----Red algae protein
Link 2: DFBPACEI2220----Laminaria japonica----Laminaria japonica proteins (LJPs)
Biological/Functional activity & target protein
ACE-inhibitory activity

The peptide exhibited strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC50 value of  2.42 μM.

Specific target protein(s) Specific Target Protein(s):
Angiotensin-converting enzyme
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(C)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(CO)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references
DFBP
[D1] DFBPANHY0661
[D2] DFBPMUFU0173
BIOPEP-UWM [D3] 7690
APD [D4] -
BioPepDB [D5] -
MBPDB [D6] -
Reference(s)
Primary literature Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501.
Other literature(s) N.D
PubDate 2016
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214