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List of peptide properties
DFBP ID - DFBPACEI1326(ACE-inhibitory peptide)
DFBP ID DFBPACEI1326
Peptide sequence KKFY
Type Native peptide
Peptide/Function name ACE-inhibitory peptide
Function-activity relationship
Main bioactivity ACE-inhibitory activity
Otheir bioactivity N.D
Calculated physicochemical properties
Three-letter amino acid Lys-Lys-Phe-Tyr
Single-letter amino acid KKFY
Peptide length 4
Peptide mass
Experimental mass Theoretical mass
585.4 Da 584.71 Da c
Net charge 0.00 c
Isoelectric point (pI) 10.18 c
IC50

15.33 uM

pIC50 -1.186
GRAVY -1.5750 c
Hydrophilic residue ratio 25% c
Peptide calculator
To calculate the physicochemical properties of bioactive peptide.
Peptide source & Food-borne protein(s) search
Classification Marine, Plant
Organism/Source Laminaria japonica
Precursor protein Laminaria japonica hydrolysates
Residue position N.D
Precursor protein(s) search
Source.1: DFBPPR1159 ---- Plant proteins ---- Replication protein A 70 kDa DNA-binding subunit B
Source.2: DFBPPR2268 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 7
Source.3: DFBPPR2716 ---- Plant proteins ---- Endoglucanase 1
Source.4: DFBPPR3189 ---- Plant proteins ---- Endoglucanase 13
Source.5: DFBPPR4833 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 56
Source.6: DFBPPR7724 ---- Plant proteins ---- Avenacosidase 2
Source.7: DFBPPR16932 ---- Animal proteins ---- Heat shock protein HSP 90-beta
Source.8: DFBPPR17050 ---- Animal proteins ---- Heat shock protein HSP 90-alpha
Source.9: DFBPPR17051 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1
Source.10: DFBPPR17442 ---- Animal proteins ---- AP-3 complex subunit beta-1
Source.11: DFBPPR17567 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL1
Source.12: DFBPPR17853 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL3
Source.13: DFBPPR18180 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL2
Source.14: DFBPPR18612 ---- Animal proteins ---- Microtubule-associated protein RP/EB family member 2
Source.15: DFBPPR18622 ---- Animal proteins ---- CYFIP-related Rac1 interactor B
Source.16: DFBPPR19535 ---- Animal proteins ---- Probable 18S rRNA (guanine-N(7))-methyltransferase
Source.17: DFBPPR20575 ---- Animal proteins ---- Peroxiredoxin-like 2A
Source.18: DFBPPR20980 ---- Animal proteins ---- Interferon-inducible GTPase 5
Source.19: DFBPPR8786 ---- Animal proteins ---- Heat shock protein HSP 90-alpha
Source.20: DFBPPR9564 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H2
Source.21: DFBPPR10043 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1
Source.22: DFBPPR10077 ---- Animal proteins ---- Heat shock protein HSP 90-alpha
Source.23: DFBPPR10393 ---- Animal proteins ---- Vitellogenin-1
Source.24: DFBPPR10958 ---- Animal proteins ---- Heat shock cognate protein HSP 90-beta
Source.25: DFBPPR12407 ---- Animal proteins ---- Heat shock protein HSP 90-beta
Source.26: DFBPPR12480 ---- Animal proteins ---- Heat shock protein HSP 90-alpha
Source.27: DFBPPR12870 ---- Animal proteins ---- Protein transport protein Sec16B
Source.28: DFBPPR13147 ---- Animal proteins ---- Heat shock protein HSP 90-beta
Source.29: DFBPPR13189 ---- Animal proteins ---- Heat shock protein HSP 90-alpha
Source.30: DFBPPR13715 ---- Animal proteins ---- Ceroid-lipofuscinosis neuronal protein 5
Source.31: DFBPPR15083 ---- Microorganism protein ---- tRNA (guanine-N(7)-)-methyltransferase
Source.32: DFBPPR15610 ---- Microorganism protein ---- Inheritance of peroxisomes protein 2
Link-research
Link 1: DFBPACEI2218----Laminaria japonica----Laminaria japonica proteins (LJPs)
Biological/Functional activity & target protein
ACE-inhibitory activity

The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC50 value of  15.33 μM.

Specific target protein(s) Specific Target Protein(s):
Angiotensin-converting enzyme
Taste properties & Structure
Bitterness
Literature report N.D
Bitter prediction tools Bitter taste prediction
SMILES N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O
Preparation method
Mode of preparation

Enzymatic hydrolysis

Enzyme(s)/starter culture

Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity
Peptide stability
Literature report: N.D
EHP-Tool: Enzymatic Hydrolysis Prediction Tool (EHP-Tool)
Peptide cytotoxicity
Literature report: N.D
Prediction: ToxinPred
Additional information
Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references
BIOPEP-UWM [D1] -
APD [D2] -
BioPepDB [D3] -
MBPDB [D4] -
Reference(s)
Primary literature Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501.
Other literature(s) N.D
PubDate 2016
Copyright © 2020. Record / license number: Chongqing ICP No. 2000214