Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1326(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1326
Peptide sequence	KKFY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Lys-Phe-Tyr

Single-letter amino acid

KKFY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
585.4 Da	584.71 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.18 ^c

IC₅₀

15.33 uM

pIC₅₀

-1.186

GRAVY

-1.5750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Plant
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1159 ---- Plant proteins ---- Replication protein A 70 kDa DNA-binding subunit B Source.2: DFBPPR2268 ---- Plant proteins ---- Probable glucomannan 4-beta-mannosyltransferase 7 Source.3: DFBPPR2716 ---- Plant proteins ---- Endoglucanase 1 Source.4: DFBPPR3189 ---- Plant proteins ---- Endoglucanase 13 Source.5: DFBPPR4833 ---- Plant proteins ---- Zinc finger CCCH domain-containing protein 56 Source.6: DFBPPR7724 ---- Plant proteins ---- Avenacosidase 2 Source.7: DFBPPR16932 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.8: DFBPPR17050 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.9: DFBPPR17051 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.10: DFBPPR17442 ---- Animal proteins ---- AP-3 complex subunit beta-1 Source.11: DFBPPR17567 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL1 Source.12: DFBPPR17853 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL3 Source.13: DFBPPR18180 ---- Animal proteins ---- [F-actin]-monooxygenase MICAL2 Source.14: DFBPPR18612 ---- Animal proteins ---- Microtubule-associated protein RP/EB family member 2 Source.15: DFBPPR18622 ---- Animal proteins ---- CYFIP-related Rac1 interactor B Source.16: DFBPPR19535 ---- Animal proteins ---- Probable 18S rRNA (guanine-N(7))-methyltransferase Source.17: DFBPPR20575 ---- Animal proteins ---- Peroxiredoxin-like 2A Source.18: DFBPPR20980 ---- Animal proteins ---- Interferon-inducible GTPase 5 Source.19: DFBPPR8786 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.20: DFBPPR9564 ---- Animal proteins ---- Inter-alpha-trypsin inhibitor heavy chain H2 Source.21: DFBPPR10043 ---- Animal proteins ---- Poly [ADP-ribose] polymerase 1 Source.22: DFBPPR10077 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.23: DFBPPR10393 ---- Animal proteins ---- Vitellogenin-1 Source.24: DFBPPR10958 ---- Animal proteins ---- Heat shock cognate protein HSP 90-beta Source.25: DFBPPR12407 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.26: DFBPPR12480 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.27: DFBPPR12870 ---- Animal proteins ---- Protein transport protein Sec16B Source.28: DFBPPR13147 ---- Animal proteins ---- Heat shock protein HSP 90-beta Source.29: DFBPPR13189 ---- Animal proteins ---- Heat shock protein HSP 90-alpha Source.30: DFBPPR13715 ---- Animal proteins ---- Ceroid-lipofuscinosis neuronal protein 5 Source.31: DFBPPR15083 ---- Microorganism protein ---- tRNA (guanine-N(7)-)-methyltransferase Source.32: DFBPPR15610 ---- Microorganism protein ---- Inheritance of peroxisomes protein 2
Link-research Inductive analysis	Link 1: DFBPACEI2218----Laminaria japonica----Laminaria japonica proteins (LJPs)

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 15.33 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501. DOI: 10.1111/jfpp.12628
Other literature(s)	N.D
PubDate	2016