Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1328(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1328
Peptide sequence	KFKY
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Lys-Phe-Lys-Tyr

Single-letter amino acid

KFKY

Peptide length

Peptide mass

Experimental mass	Theoretical mass
585.5 Da	584.71 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

10.18 ^c

IC₅₀

10.73 uM

pIC₅₀

-1.031

GRAVY

-1.5750^c

Hydrophilic residue ratio

25%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Marine, Plant
Organism/Source	Laminaria japonica
Precursor protein	Laminaria japonica hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR1775 ---- Plant proteins ---- B3 domain-containing protein IDEF1 Source.2: DFBPPR3540 ---- Plant proteins ---- Auxin transporter-like protein 2 Source.3: DFBPPR3610 ---- Plant proteins ---- Auxin transporter-like protein 1 Source.4: DFBPPR8370 ---- Plant proteins ---- Maturase K Source.5: DFBPPR16068 ---- Animal proteins ---- Cytochrome P450 2E1 Source.6: DFBPPR16132 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11C Source.7: DFBPPR16135 ---- Animal proteins ---- Gap junction alpha-1 protein Source.8: DFBPPR16175 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11A Source.9: DFBPPR16559 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.10: DFBPPR16732 ---- Animal proteins ---- HORMA domain-containing protein 2 Source.11: DFBPPR17098 ---- Animal proteins ---- Cytochrome P450 2E1 Source.12: DFBPPR17448 ---- Animal proteins ---- Gap junction alpha-1 protein Source.13: DFBPPR18548 ---- Animal proteins ---- Lipoyl synthase, mitochondrial Source.14: DFBPPR18804 ---- Animal proteins ---- Importin subunit alpha-8 Source.15: DFBPPR19039 ---- Animal proteins ---- Signal peptidase complex catalytic subunit SEC11A Source.16: DFBPPR19506 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.17: DFBPPR21259 ---- Animal proteins ---- Elongation factor 1-delta Source.18: DFBPPR21316 ---- Animal proteins ---- Translation initiation factor eIF-2B subunit alpha Source.19: DFBPPR21564 ---- Animal proteins ---- HORMA domain-containing protein 2 Source.20: DFBPPR22593 ---- Animal proteins ---- UPF0688 protein C1orf174 homolog Source.21: DFBPPR8757 ---- Animal proteins ---- Gap junction alpha-1 protein Source.22: DFBPPR8902 ---- Animal proteins ---- Cytochrome P450 2E1 Source.23: DFBPPR8984 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.24: DFBPPR9625 ---- Animal proteins ---- HORMA domain-containing protein 1 Source.25: DFBPPR9634 ---- Animal proteins ---- NADH-ubiquinone oxidoreductase chain 5 Source.26: DFBPPR9783 ---- Animal proteins ---- Importin subunit alpha-8 Source.27: DFBPPR10690 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.28: DFBPPR11024 ---- Animal proteins ---- Gap junction alpha-1 protein Source.29: DFBPPR11999 ---- Animal proteins ---- Dymeclin Source.30: DFBPPR12108 ---- Animal proteins ---- Protein strawberry notch homolog 1 Source.31: DFBPPR12353 ---- Animal proteins ---- Cytochrome P450 2E1 Source.32: DFBPPR12399 ---- Animal proteins ---- Gap junction alpha-1 protein Source.33: DFBPPR12494 ---- Animal proteins ---- V(D)J recombination-activating protein 1 Source.34: DFBPPR12717 ---- Animal proteins ---- Phosphorylase b kinase regulatory subunit alpha, liver isoform Source.35: DFBPPR13047 ---- Animal proteins ---- Elongation factor 1-delta Source.36: DFBPPR13058 ---- Animal proteins ---- Anion exchange transporter Source.37: DFBPPR13961 ---- Animal proteins ---- Elongation factor 1-delta Source.38: DFBPPR14891 ---- Microorganism protein ---- DNA polymerase epsilon catalytic subunit A Source.39: DFBPPR14966 ---- Microorganism protein ---- PAN2-PAN3 deadenylation complex catalytic subunit PAN2 Source.40: DFBPPR15567 ---- Microorganism protein ---- Mating-type protein A2
Link-research Inductive analysis	Link 1: DFBPACEI2217----Laminaria japonica----Laminaria japonica proteins (LJPs)

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited potent Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 10.73 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Bitter taste ^prediction

SMILES

N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccccc1)C(=O)N[C@@]([H])(CCCCN)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	Laminaria japonica protein was hydrolyzed with combined enzymes (alcalase, trypsin and papain).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

(1) Laminaria japonica proteins would be a good source of hypotensive peptides.
(2) A simple and efficient RP-HPLC method was developed for simultaneous analysis of eight small anti-hypertensive peptides with tyrosine at the C-terminal.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Chen, J.-C., Wang, J., Zheng, B.-D., Pang, J., Chen, L.-J., Lin, H.-t., et al. Simultaneous Determination of 8 Small Antihypertensive Peptides with Tyrosine at the C-Terminal inLaminaria japonicaHydrolysates by RP-HPLC Method. Journal of Food Processing and Preservation. 2016, 40, 492-501. DOI: 10.1111/jfpp.12628
Other literature(s)	N.D
PubDate	2016