Peptide properties

1Top 2Function-activity relationship 3Calculated physicochemical properties 4Peptide source & Food-borne protein(s) search 5Biological/Functional activity & target protein 6Taste properties & Structure 7Preparation method 8Stability & Cytotoxicity 9Additional information 10Database cross-references 11Reference(s)

List of peptide properties

DFBP ID - DFBPACEI1368(ACE-inhibitory peptide)

DFBP ID	DFBPACEI1368
Peptide sequence	DYVGN
Type	Native peptide
Peptide/Function name	ACE-inhibitory peptide

Function-activity relationship

Main bioactivity	ACE-inhibitory activity
Otheir bioactivity	N.D

Calculated physicochemical properties

Three-letter amino acid

Asp-Tyr-Val-Gly-Asn

Single-letter amino acid

DYVGN

Peptide length

Peptide mass

Experimental mass	Theoretical mass
N.D	566.57 Da ^c

Net charge

0.00 ^c

Isoelectric point (pI)

3.13 ^c

IC₅₀

0.72 uM

pIC₅₀

0.143

GRAVY

-0.9000^c

Hydrophilic residue ratio

40%^c

Peptide calculator

Peptide source & Food-borne protein(s) search

Classification	Plant
Organism/Source	Wheat
Precursor protein	Wheat germ hydrolysates
Residue position	N.D
Precursor protein(s) search	Source.1: DFBPPR6782 ---- Plant proteins ---- 1-Cys peroxiredoxin PER1 Source.2: DFBPPR7049 ---- Plant proteins ---- 1-Cys peroxiredoxin PER1 Source.3: DFBPPR18592 ---- Animal proteins ---- Alpha-1-antiproteinase Source.4: DFBPPR21857 ---- Animal proteins ---- Ankyrin repeat and SOCS box protein 2 Source.5: DFBPPR9173 ---- Animal proteins ---- Neurotrophin-3 Source.6: DFBPPR12822 ---- Animal proteins ---- Corticosteroid-binding globulin Source.7: DFBPPR13706 ---- Animal proteins ---- Alpha-1-antiproteinase
Link-research Inductive analysis	There are no literature reports on the discovery of this sequence in other food-source proteins.

Biological/Functional activity & target protein

ACE-inhibitory activity	The peptide exhibited very strong Angiotensin-Converting Enzyme (ACE) (EC 3.4.15.1) inhibitory activity with an IC₅₀ value of 0.72 μM.
Specific target protein(s)	Specific Target Protein(s): Angiotensin-converting enzyme

Taste properties & Structure

Bitterness

Literature report	N.D
Bitter prediction tools	Non-bitter taste ^prediction

SMILES

N[C@@]([H])(CC(=O)O)C(=O)N[C@@]([H])(Cc1ccc(O)cc1)C(=O)N[C@@]([H])(C(C)C)C(=O)NCC(=O)N[C@@]([H])(CC(=O)N)C(=O)O

Preparation method

Mode of preparation	Enzymatic hydrolysis
Enzyme(s)/starter culture	The hydrolyzate with the most potent ACE inhibitory activity was obtained by 0.5 wt.%–8 h Bacillus licheniformis alkaline protease hydrolysis after 3.0 wt.%–3 h α-amylase treatment of defatted WG (IC₅₀: 0.37 mg protein ml⁻¹).

Stability & Cytotoxicity

Peptide stability

Literature report:	N.D
EHP-Tool:	Enzymatic Hydrolysis Prediction Tool (EHP-Tool)

Peptide cytotoxicity

Literature report:	N.D
Prediction:	ToxinPred

Additional information

The purified peptide DYVGN is a possible candidate for application as a dietary supplement or as a component of a functional food product.

Database cross-references

[D1]

[D2]

[D3]

[D4]

Reference(s)

Primary literature	Matsui T, Li CH, Osajima Y. Preparation and characterization of novel bioactive peptides responsible for angiotensin I-converting enzyme inhibition from wheat germ. J Pept Sci. 1999 Jul;5(7):289-97. PMID: 10442764 DOI: 10.1002/(SICI)1099-1387(199907)5:7<289::AID-PSC196>3.0.CO;2-6
Other literature(s)	N.D
PubDate	1999